Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMP-8 (collagenase-2, neutrophil collagenase) is involved in quite a few diseases such as cancer, arthritis and asthma. The SensoLyte® 490 MMP-8 Assay Kit is a complete assay system designed to continuously analyze MMP activities or to screen MMP-8 inhibitors by using a fluorogenic MMP-8 substrate.
Component A: MMP-8 substrate Ex/Em=340 nm/490 nm upon cleavage: 270 µL
Component B: EDANS, fluorescence reference standard Ex/Em=340 nm/490 nm: 1 mM, 10 µL
Component C: APMA, 4-aminophenylmercuric acetate (Caution: Toxic! Handle with care): 1 M, 100 µL
Component D: Assay buffer: 60 mL
Component E: Stop solution: 30 mL
Storage & stability
Store all components at -20°C. Protect components A and B from light. Components D and E can be stored at 4 °C for convenience.