Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMP-9 (gelatinase-B, collagenase-IV) is involved in quite a few diseases such as a cancer, angiogenesis, alopecia and metastasis. The SensoLyte® 490 MMP-9 Assay Kit is a complete assay system designed to continuously analyze MMP activity or to screen for MMP-9 inhibitors using a fluorogenic MMP-9 substrate. The assays are performed in a convenient 96-well or 384-well microplate format. The kit provides speed, convenience and accuracy in MMP-9 analysis. Members of the MMP family have poor substrate sequence specificity, making it difficult to use a peptide substrate alone to differentiate the activity of a particular MMP from other MMPs. If several MMPs are coexisting in your samples and you would like to specifically measure MMP-9 activity, please choose the SensoLyte® Plus MMP-9 Assay Kit, Cat# AS-72017.
Component A: MMP-9 substrate Ex/Em=340 nm/490 nm 270 µL
Component B: EDANS, fluorescence reference standard Ex/Em=340 nm/490 nm: 1 mM DMSO solution, 10 µL
Component C: APMA, 4-aminophenylmercuric acetate (Caution: Toxic! Handle with care): 1 M, 100 µL
Component D: Assay buffer: 60 mL
Component E: Stop solution: 30 mL
Storage & stability
Store all components at -20°C. Protect Components A and B from light. Components D and E can be stored at 4°C for convenience.