Assay-kits

SensoLyte® 520 ß-Secretase (BACE1) Activity Assay Kit Fluorimetric - 1 kit

465,00
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  • Cat.Number : AS-71144
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    In stock
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β-Secretase (BACE1) is a membrane-anchored aspartic protease existing in acidic subcellular vesicles. It is a key player in producing β-amyloid peptides through proteolytic cleavage of the β-amyloid precursor protein (APP). Accumulation of neurotoxic β-amyloid peptide is seen in senile plaques in the brains of patients with Alzheimer’s disease (AD), an age-related cognitive disorder. Therefore, targeting β-secretase is considered a potential therapeutic strategy for the prevention and treatment of AD.
The SensoLyte® 520 β-Secretase Assay Kit is a homogeneous assay that can be used to detect the activity of enzyme and for screening of secretase inhibitors. It contains a QXL® 520/ HiLyte™ Fluor 488 FRET substrate. The sequence of this FRET peptide is derived from the β-secretase cleavage site on the Swedish APP mutation. This mutation causes the enhancement of β-secretase to process APP, resulting in an early onset of AD. Active β-secretase cleaves FRET substrate into two separate fragments resulting in the release of HiLyte™ Fluor 488 fluorescence that can be monitored at excitation/emission= 490/520 nm. The long wavelength fluorescence of HiLyte™ Fluor 488 is less interfered by the autofluorescence of components in biological samples and test compounds.

Specifications

Packaging
Kits components
  • Component A: HiLyte™ Fluor 488/QXL™-520 BACE1 substrate, Ex/Em=490/520 nm upon cleavage: 60µL Component B: HiLyte™ Fluor 488, fluorescence reference standard 1 mM DMSO solution: 20 µL Component C: BACE1 Inhibitor 250 µM: 10 µL Component D: 2X Assay buffer: 20 mL Component E: Stop Solution: 10 mL Component F: Human BACE1 enzyme: 20 µL
Chemistry
UniProt number
  • P56817
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except for Component F. Store b-secretase (Component F) at –80°C. Protect Components A and B from light and from moisture. Components D and E can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Inhibiting β-secretase activity in Alzheimer's disease cell models with single-chain antibodies specifically targeting APP

J Mol Biol . 2011 Jan 14 ; 405 436 | DOI : 10.1016/j.jmb.2010.10.054

  • S. Boddapati
  • et al

Promotion of BACE1 mRNA alternative splicing reduces amyloid β-peptide production

J Biol Chem . 2008 Jul 04 ; 283 18694 | DOI : 10.1074/jbc.M801322200

  • K.R. Mowrer
  • M.S. Wolfe