Assay-kits

SensoLyte® 520 ADAMTS13 Activity Assay Kit Fluorimetric - 1 kit

518,00
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  • Cat.Number : AS-72232
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Quantity

ADAMTS13 (A Disintegrin-like and Metalloprotease with Thrombospondin type-1 motifs 13) is a metalloproteinase that is primarily synthesized in the liver cells and secreted into blood circulation. ADAMTS13 specifically cleaves von Willebrand factor (vWF) at the Tyr1605-Met1606 peptide bond. vWF is a multimeric glycoprotein that mediates adhesion and aggregation of platelets. ADAMTS13 is essential for preventing platelet aggregation in the circulation. Deficiency of plasma ADAMTS13 activity may lead to thrombotic thrombocytopenic purpura (TTP), a potentially lethal syndrome characterized by the formation of VWF-platelet-rich thrombi in the arterioles and capillaries. Studies also have shown that ADAMTS13 is involved in angiogenesis, inflammation, atherosclerosis, and liver cirrhosis. Measurement of ADAMTS13 activity in plasma has been used for supporting the acute diagnosis of TTP.
The SensoLyte®520 ADAMTS13 Assay Kit employs an internally quenched vWF73 FRET peptide substrate for the detection of enzyme activity. This substrate contains the 5-FAM/QXL® 520 FRET
pair. ADAMTS13 cleaves this FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence, which can be monitored at Ex/Em= 490/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples.

Specifications

Packaging
Kits components
  • Component A: 5-FAM/QXL® 520 ADAMTS13 substrate, Ex/Em=490/520 nm upon cleavage: 50µL Component B: 5-FAM, fluorescence reference standard, Ex/Em=490/520 nm: 0.1 mM, 15 µL Component C: Recombinant human ADAMTS13: 50µg/mL, 20 µL Component D: 2X Assay Buffer: 30 mL Component E: ADAMTS13 Inhibitor: 1 M, 15 µL
Chemistry
UniProt number
  • Q76LX8
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except for Component C; store Component C at -80°C; protect Components A and B from light and moisture; Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 10 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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