Assay-kits

SensoLyte® 520 Cathepsin D Assay Kit Fluorimetric - 1 kit

443,00
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  • Cat.Number : AS-72170
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown. Cathepsin D is involved in the pathogenesis of several diseases such as breast cancer and Alzheimer disease.
The SensoLyte® 520 Cathepsin D Activity Assay Kit uses a QXL™ 520/HiLyte™ Fluor 488 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, which is optimal for cathepsin activity. The kit can be used to detect the activity of Cathepsin D enzyme in biological samples and purified enzyme preparations.

Specifications

Packaging
Kits components
  • Component A: QXL™ 520/HiLyte™ Fluor 488, Cathepsin D substrate, Ex/Em=490 nm/520 nm upon cleavage: 1 mM, 50 µL Component B: HiLyte™ Fluor 488, fluorescence reference standard, Ex/Em=490 nm/520 nm: 1 mM, 10 µL Component C: Cathepsin D, bovine spleen: 0.1 mg/mL, 10 µL Component D: Assay Buffer: 20 mL Component E: Pepstatin A: 100 µM, 20 µL Component F: DTT: 1 M, 100 µL
Chemistry
UniProt number
  • P07339
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except for Component C. Store Component C at -80°C. Protect Components A and B from light and moisture. Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 0.78 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

High-Fat Diet–Induced Adipocyte Cell Death Occurs Through a Cyclophilin D Intrinsic Signaling Pathway Independent of Adipose Tissue Inflammation

Diabetes . 2011 Jul 06 ; 60 2134 | DOI : 10.2337/db10-1411

  • D. Feng
  • et al

Functional Heterogeneity of CD11c-positive Adipose Tissue Macrophages in Diet-induced Obese Mice

J Biol Chem . 2010 Mar 22 ; 285 15333 | DOI : 10.1074/jbc.M110.100263

  • P.P. Li
  • et al

Bovine cathepsin D activity under high pressure

Food Chem . 2010 May 15 ; 120 474 | DOI : https://doi.org/10.1016/j.foodchem.2009.10.040

  • R. Buckow
  • et al