Assay-kits

SensoLyte® 520 Deubiquitination Assay Kit Fluorimetric - 1 kit

364,00
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  • Cat.Number : AS-72203
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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Deubiquitinating enzymes (DUBs) are proteases that reverse ubiquitin modifications and can rescue a protein from proteasomal degradation. DUBs specifically cleave ubiquitin-linked molecules downstream of the terminal carbonyl of the last residue of ubiquitin (Gly76). They are classified in five families. Well-defined families include ubiquitin carboxyl-terminal hydrolases (UCH) and ubiquitin-processing proteases (UBP). DUBs act as regulators of diverse cellular processes, such as development, growth and DNA repair. They have become actively studied molecular targets for drug discovery since they are implicated in the pathogenesis of many human diseases.
The SensoLyte® 520 Deubiquitination Assay Kit provides a convenient assay for the screening of enzyme inhibitors and activators or for continuous assay of deubiquitination activity through the use of a fluorogenic peptide substrate. Upon the cleavage by a deubiquitin protease, this substrate generates the green fluorophore that can be detected at excitation/emission=490/520 nm.

Specifications

Packaging
Kits components
  • Component A: 520 DUB fluorogenic substrate, Ex/Em=490 nm/520 nm upon cleavage: 2 mM, 50 µL Component B: Fluorescence reference standard, Ex/Em=490 nm/520 nm: 2 mM, 10 µL Component C: Deubiquitin protease, Recombinant Human UCH-L3: 0.5 mg/mL, 20 µL Component D: 2X Assay Buffer: 25 mL Component E: DTT: 1 M, 100 µL Component F: UCH-L3 Inhibitor: 4 mM, 10 µL
Chemistry
UniProt number
  • P15374
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except for Component C. Store Component C at -80°C. Protect Components A and B from light and moisture. Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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