SensoLyte® 520 HDAC Activity Assay Kit Fluorimetric - 1 kit

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  • Cat.Number : AS-72084
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied


Histone deacetylase (HDAC) enzymes modulate gene expression through the deacetylation of lysine residues on histone proteins and act as transcriptional repressors of genes. Based on their role in cell cycling, apoptosis and differentiation, HDACs have been chosen as therapeutic targets for the treatment of cancer and neurodegenerative diseases.
The SensoLyte® 520 HDAC Activity Assay Kit provides a convenient, two-step homogeneous procedure for measuring HDAC activity using a fluorogenic substrate. In the first step, an acetylated substrate is incubated with HDAC containing samples. Deacetylation of substrate sensitizes it, so that, in the second step mixing with the HDAC developer generates a fluorophore, which can then be detected at Ex/Em= 490 nm/520 nm. The substrate included in the kit is cell-permeable, and the assay can be used to measure HDAC activity directly in cell culture in a 96-well plate without a time-consuming cell extraction step. This kit also can be used for high throughput screening of HDAC inhibitors using extracts or purified enzymes. The long wavelength fluorescence of HDAC 520 substrate is less interfered by the autofluorescence of cell components and test compounds.


Kits components
  • Component A: HDAC 520 substrate, Ex/Em=490 nm/520 nm: 25 mM in DMSO, 40 µL Component B: Deacetylated 520 standard, Ex/Em=490 nm/520 nm: 25 mM in DMSO, 10 µL Component C: HeLa Nuclear Extract: ~ 4 mg protein/mL, 15 µL Component D: Assay Buffer: 20 mL Component E: Trichostatin A (Inhibitor of HDAC): 300 µM in DMSO, 100 µL Component F: HDAC Developer (10X): 0.5 mL Component G: Cell Lysis Buffer: 20 mL
Sequence one letter code
  • Ac-RGK(Ac)-AMC
Sequence three letter code
  • Ac-Arg-Gly-Lys(Ac)-AMC
CAS registry number
  • 660846-97-9
UniProt number
  • Q13547
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component C at -80°C. Store all other components at -20°C. Components D and G can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Biomarker Target
Detection Method
Detection Limit
  • 0.78 ug/ml
Research Area
Sub-category Research Area
  • Research use
Code Nacres
  • NA.32

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Histone Deacetylase Inhibitors Prolong Cardiac Repolarization through Transcriptional Mechanisms.

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  • et al

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Invest New Drugs . 2011 Oct 08 ; 30(5) 1887 | DOI : 10.1007/s10637-011-9752-z

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  • et al