Assay-kits

SensoLyte® HAT (p300) Assay Kit Fluorimetric - 1 kit

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  • Cat.Number : AS-72172
  • Availability :
    In stock
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Histone acetyltransferases (HATs) enzymes regulate the acetylation of histones and non-histone proteins. The acetylation of the e-amino groups of lysine residues present at histone tails correlates largely with transcriptional activation, but it is also involved in DNA replication, DNA repair and protein–protein interactions. HATs play major roles in the control of cell fate and their misregulation is implicated in the development of some human tumors. HAT p300 is a transcriptional coactivator that acetylates core histones facilitating chromatin decondensation and recruiting basic RNA polymerase machinery.
The SensoLyte® HAT (p300) Assay Kit provides a convenient protocol for screening of enzyme inhibitors and for continuous assay of p300 activity. For added convenience, this kit includes two peptide substrates: histone H3 (1-21) peptide and non-histone p53 peptide (368-386). After incubation with acetyl CoA and the substrate, the p300 enzyme generates acetylated H3 or p53 peptide and CoASH. The thiol groups of CoASH can be detected with fluorogenic reagent at excitation/ emission=389nm/513nm.

Specifications

Packaging
Kits components
  • Component A: Acetyl CoA: 200 µL Component B: Histone H3 peptide (1-21): 1 mM, 200 µL Component C: p53 peptide (368-386): 4 mM, 200 µL Component D: HAT, Recombinant p300 enzyme: 100 µL, 0.1 mg/mL Component E: Assay Buffer: 40 mL Component F: p300 Inhibitor: 10 mM, 10 µL Component G: p300 Developer: 400 µL Component H: Stop Solution: 10 mL Component I: CoASH standard: 5 mM, 10 µL
Chemistry
UniProt number
  • Q09472
Properties
Absorbance (nm)
  • 389
Emission (nm)
  • 513
Storage & stability
Storage Conditions
  • Store all kit components, except Component D, at -20°C. Store Component D at -80°C. Aliquot as needed to avoid multiple freeze-thaw cycles. Protect Component G from light and moisture. Components E and H can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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