SensoLyte® 520 HIV Protease Assay Kit Fluorimetric - 1 kit

Check your price
  • Cat.Number : AS-71147
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Alternative choices


The SensoLyte® 520 HIV Assay Kit uses a new FRET peptide substrate that incorporates HiLyte™ Fluor 488 (fluorophore) and QXL™ 520 (quencher) for continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by HIV protease, the fluorescence of HiLyte™ Fluor 488 is recovered and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this HiLyte™ Fluor 488/QXL™ 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components, thus providing better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.


Kits components
  • Component A: HIV-1 protease FRET substrate: 120 µL Component B: HiLyte Fluor™488, fluorescence reference standard Ex/Em=490 nm/520 nm: 100 µM, 5 µL Component C: Pepstatin A, a characterized HIV-1 protease inhibitor: 27.4 µg powder Component D: 2X Assay buffer 20 mL Component E: Stop solution: 10 mL Component F: DMSO: 50 µL Component G: DTT: 1 M, 150 µL
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all components at -20°C. Components E and F can be stored at room temperature for convenience. Protect Components A and B from light.
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
  • Research use
Code Nacres
  • NA.32

You may also be interested in the following product(s)

Tube of HIV Protease FRET SubstrateI peptide

HIV Protease FRET Substrate I - 1 mg

Cat.Number : AS-22992
283.00 Excl. Tax
Tube of HIV Substrate peptide, HF488

HIV Substrate, HiLyte™ Fluor 488 - 0.1 mg

Cat.Number : AS-60635
147.00 Excl. Tax
Vial of HIV-1 protease, recombinant protein

HIV-1 protease, recombinant - 5 µg

Cat.Number : AS-72028-5
432.00 Excl. Tax


Screening of HIV-1 protease using a combination of an ultra-high-throughput fluorescent-based assay and RapidFire mass spectrometry

J Biomol Screen . 2015 Feb 13 ; 20(5) 606 | DOI : 10.1177/1087057115570838

  • J. Meng
  • et al

Design, asymmetric synthesis, and evaluation of pseudosymmetric sulfoximine inhibitors against HIV-1 protease

J BMC . 2010 Mar 01 ; 18(5) 2037 | DOI :

  • D. Lu
  • et al

Synthesis of dammarane-type triterpene derivatives and their ability to inhibit HIV and HCV proteases

Bioorg Med Chem . 2009 Mar 14 ; 17(8) 3003 | DOI : 10.1016/j.bmc.2009.03.019

  • Y. Wei
  • et al