Assay-kits

SensoLyte® 520 Sortase A Activity Assay Kit Fluorimetric - 1 kit

462,00
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  • Cat.Number : AS-72228
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Sortases are a family of membrane–anchored transpeptidases expressed by Gram–positive bacteria. Sortase A catalyzes the cleavage of C-terminal recognition motif (LPXTG) of multiple structurally unrelated proteins followed by formation of an amide bond with the peptidoglycan layer of the bacteria. Since sortases are widely distributed among a variety of bacterial pathogens and required for virulence, they represent a promising therapeutic target for the development of novel anti-infective agents. In addition, Sortase A can be used as a biotechnology tool for a variety of protein modifications and immobilization by sortase-mediated protein ligation.
The SensoLyte® 520 Sortase A Assay Kit employs a novel internally quenched 5-FAM/QXL® FRET substrate for the detection of protease activity. The enzyme cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence, which can be monitored at excitation/emission= 490/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds.

Specifications

Packaging
Kits components
  • Component A: 5-FAM/QXL™520 Sortase substrate, Ex/Em=490/520 nm upon cleavage: 0.4 mM, 50 µL Component B: 5-FAM, fluorescence reference standard, Ex/Em=490/520 nm: 0.4 mM, 15 µL Component C: Sortase A protease, Recombinant: 0.05 mg/mL, 200 µL Component D: 2X Assay Buffer: 30 mL Component E: Sortase Inhibitor: 10 mM, 15 µL
Chemistry
UniProt number
  • Q2FV99
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except for Component C. Store Component C at -80°C. Protect Components A and B from light and moisture. Component D can be stored at room temperature for convenience
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 20 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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