Peptides

Bacterial Sortase Substrate I, FRET - 1 mg

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  • Cat.Number : AS-62231
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    In stock

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This 5-amino acid peptide is a sortase substrate, C-terminal sorting signal. Sortase cleaves surface proteins at the LPXTG motif and catalyzes the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Sortases are a family of Gram-positive transpeptidases responsible for anchoring surface protein virulence factors to the peptidoglycan cell wall layer. Cleavage of this FRET substrate by sortase reveals the fluorescent signal, Abs/Em = 340/490 nm.

Specifications

Chemistry
Sequence one letter code
  • DABCYL-LPETG-EDANS
Sequence three letter code
  • DABCYL-Leu-Pro-Glu-Thr-Gly-EDANS
Molecular Mass/ Weight
  • 1015.2
Properties
Absorbance (nm)
  • 340
Emission (nm)
  • 490
Modification
Conjugation type
  • Double dyes
Modification Name
Conjugation
  • Conjugated
Quantity & Purity
Purity
  • ≥ 95%
Storage & stability
Form
  • Lyophilized
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • bacteria

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