Assay-kits

SensoLyte® 520 Thrombin Activity Assay Kit Fluorimetric - 1 kit

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  • Cat.Number : AS-72129
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    In stock
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Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation. Thrombin also influences a number of normal and pathological processes, including inflammation, tissue repair, embryogenesis, angiogenesis, and tumor invasion. The SensoLyte® 520 Thrombin Assay Kit is optimized for detecting thrombin activity. This kit contains a novel internally quenched 5-FAM/QXL™ 520 FRET substrate for thrombin. Thrombin cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence which can be monitored at excitation/emission= 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assay can be performed in a convenient 96-well or 384-well microplate format for screening of enzyme inhibitors.

Specifications

Packaging
Kits components
  • Component A: 5-FAM/QXL™520 Thrombin substrate, Ex/Em=490 nm/520 nm upon cleavage: 2 mM, 50 µL Component B: 5-FAM, Fluorescence reference standard, Ex/Em=490 nm/520 nm: 2 mM, 10 µL Component C: 2X Assay buffe:r 20 mL Component D: Purified human thrombin enzyme: 0.1 mg/mL, 40 µL Component E: Thrombin inhibitor: 60 µM, 10 µL Component F: Stop solution: 5 mL
Chemistry
UniProt number
  • P00734
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components, except Component D, at -20°C. Store Component D at -80°C. Aliquot as needed to avoid multiple freeze-thaw cycles. Protect Components A and B from light and moisture. Components C and F can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 6.25 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

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Ion-Responsive Hemin–G-Quadruplexes for Switchable DNAzyme and Enzyme Functions

Chemistry . 2014 May 05 ; 20 5619 | DOI : 10.1002/chem.201304702

  • M.A. Aleman-Garcia

Effect of thrombin on human amnion mesenchymal cells, mouse fetal membranes, and preterm birth

J Biol Chem . 2014 May 09 ; 289 13295 | DOI : 10.1074/jbc.M114.550541

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  • et al

System-level multi-target drug discovery from natural products with applications to cardiovascular diseases

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  • et al

Antimicrobial and Anticoagulant Activities of N-Chlorotaurine, N,N-Dichloro-2,2-Dimethyltaurine, and N-Monochloro-2,2-Dimethyltaurine in Human Blood

Antimicrob Agents Chemother . 2012 Apr 01 ; 56 1979 | DOI : 10.1128/AAC.05685-11

  • C. Martini
  • et al

High thrombin concentrations in fibrin sealants induce apoptosis in human keratinocytes

J Biomed Mater Res A . 2012 May 01 ; 100 1239 | DOI : 10.1002/jbm.a.34007

  • A. Gugerell
  • et al

Layer-by-layer co-immobilization of soluble complement receptor 1 and heparin on islets

Biomaterials . 2011 Sep 01 ; 32 6487 | DOI : 10.1016/j.biomaterials.2011.05.048

  • N.M. Luan
  • et al

Immobilization of anticoagulant-loaded liposomes on cell surfaces by DNA hybridization

Biomaterials . 2011 Jan 11 ; 32 797 | DOI : 10.1016/j.biomaterials.2011.07.002

  • H. Chen
  • et al

Aptamer-conjugated nanoparticles efficiently control the activity of thrombin

Adv Func Materials . 2010 Sep 14 ; 20 3175 | DOI : https://doi.org/10.1002/adfm.201000642

  • Y.C. Shiang
  • et al