Assay-kits

SensoLyte® ADHP Peroxidase Assay Kit Fluorimetric - 1 kit

194,00
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  • Cat.Number : AS-71111
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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Quantity

HRP conjugates are extensively used as secondary detection reagents in ELISAs, immuno-histochemical techniques and Northern, Southern and Western blot analyses. SensoLyte® ADHP Peroxidase Assay Kit uses highly purified ADHP to quantify peroxidase activity in solutions, in cell extracts and in live cells and on solid surfaces (such as PVDF membranes). The kit can be used for characterizing kinetics of enzyme reaction and high throughput screening of oxidase inhibitors. The kit contains: ADHP peroxidase substrate (Ex/Em=573/590 nm upon oxidation), Calibration standard, Reaction buffer, Stop buffer, An optimized ‘mix and read’ assay protocol that is compatible with HTS liquid handling instruments

Specifications

Packaging
Kits components
  • Component A: ADHP: 10 mM, 250 µL Component B: H2O2: 1 vial Component C: Assay buffer: 60 mL
Properties
Absorbance (nm)
  • 573
Emission (nm)
  • 590
Storage & stability
Storage Conditions
  • Store Component A at -20°C and keep from direct light. Store the rest of the components at 4°C.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32