Assay-kits

SensoLyte® AFC Thrombin Activity Assay Kit Fluorimetric - 1 kit

401,00
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  • Cat.Number : AS-72130
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    In stock
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Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation. Thrombin also influences a number of normal and pathological processes, including inflammation, tissue repair, embryogenesis, angiogenesis, and tumor invasion.
The SensoLyte® AFC Thrombin Assay Kit is optimized for screening of enzyme inhibitors. This kit contains a fluorogenic substrate which shows high reactivity and low background. Thrombin cleaves the substrate resulting in the release of the AFC (7-amido-4-trifluoromethylcoumarin) fluorophore. Fluorescence can be monitored at excitation/emission= 380 nm/500 nm. The assays are performed in a convenient 96-well or 384-well microplate format.

Specifications

Packaging
Kits components
  • Component A: AFC Thrombin substrate, Ex/Em=380 nm/500 nm: 5 mM, 50 µL Component B: AFC, Fluorescence reference standard, Ex/Em=380 nm/500 nm: 5 mM, 10 µL Component C: 2X Assay buffer: 20 mL Component D: Purified human thrombin enzyme: 0.01 mg/mL, 40 µL Component E: Thrombin inhibito:r 10 mM, 10 µL Component F: Stop solution: 5 mL
Chemistry
UniProt number
  • P00734
Properties
Absorbance (nm)
  • 380
Emission (nm)
  • 500
Storage & stability
Storage Conditions
  • Store all kit components, except Component D, at -20°C. Store Component D at -80°C. Aliquot as needed to avoid multiple freeze-thaw cycles. Protect Components A and B from light and moisture. Components C and F can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Reactive Center Loop (RCL) Peptides Derived from Serpins Display Independent Coagulation and Immune Modulating Activities.

J Biol Chem . 2015 Nov 30 ; 291(6) 2874 | DOI : 10.1074/jbc.M115.704841

  • S. Ambadapadi
  • et al

Interactions of some commonly used drugs with Human α-thrombin

J Biomol Struct Dyn . 2014 Jun 19 ; 33(5) 1008 | DOI : 10.1080/07391102.2014.923329

  • D.G. Nair
  • et al

Process-scale purification and analytical characterization of highly gamma-carboxylated recombinant human prothrombin

J Chromatogr A . 2013 Dec 15 ; 1325 171 | DOI : 10.1016/j.chroma.2013.12.024

  • M. Wendeler
  • et al

Interleukin-1 promotes coagulation, which Is necessary for protective immunity in the lung against Streptococcus pneumoniae infection

J Infect Dis . 2013 Jan 01 ; 207(1) 50 | DOI : 10.1093/infdis/jis651

  • H. Yang
  • et al

Intravenous and oral administrations of DD2 [7-Amino-2-

Thromb Res . 2012 Jul 12 ; 130(4) e222 | DOI : 10.1016/j.thromres.2012.06.018

  • T. Sasaki
  • et al

Novel agmatine and agmatine-like peptidomimetic inhibitors of the West Nile Virus NS2B/NS3 serine protease

Eur J Med Chem . 2011 Apr 28 ; 46(7) 3130 | DOI : 10.1016/j.ejmech.2011.04.055

  • H.A. Lim
  • et al