SensoLyte® AFC Thrombin Activity Assay Kit Fluorimetric - 1 kit

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  • Cat.Number : AS-72130
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation. Thrombin also influences a number of normal and pathological processes, including inflammation, tissue repair, embryogenesis, angiogenesis, and tumor invasion.
The SensoLyte® AFC Thrombin Assay Kit is optimized for screening of enzyme inhibitors. This kit contains a fluorogenic substrate which shows high reactivity and low background. Thrombin cleaves the substrate resulting in the release of the AFC (7-amido-4-trifluoromethylcoumarin) fluorophore. Fluorescence can be monitored at excitation/emission= 380 nm/500 nm. The assays are performed in a convenient 96-well or 384-well microplate format.


Kits components
  • Component A: AFC Thrombin substrate, Ex/Em=380 nm/500 nm: 5 mM, 50 µL Component B: AFC, Fluorescence reference standard, Ex/Em=380 nm/500 nm: 5 mM, 10 µL Component C: 2X Assay buffer: 20 mL Component D: Purified human thrombin enzyme: 0.01 mg/mL, 40 µL Component E: Thrombin inhibito:r 10 mM, 10 µL Component F: Stop solution: 5 mL
UniProt number
  • P00734
Absorbance (nm)
  • 380
Emission (nm)
  • 500
Storage & stability
Storage Conditions
  • Store component D at -80°C. Store all other components at -20°C. Component C and F can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
  • Research use
Code Nacres
  • NA.32

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