Assay-kits

SensoLyte® AMC Tissue-type (tPA) Activity Assay Kit Fluorimetric - 1 kit

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  • Cat.Number : AS-72160
  • Availability :
    In production
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Tissue-type plasminogen activator (tPA) is a serine protease that cleaves pro-enzyme plasminogen into active plasmin. It has been recognized that tPA play important roles in various physiological and pathological processes. In plasma, it functions as a thrombolytic enzyme and can be used for treatment of ischemic stroke. In the central nervous system, tPA promotes events associated with synaptic plasticity and cell death in a number of settings, such as cerebral ischemia and seizures.
The SensoLyte AMC tPA Activity Assay Kit is optimized for assaying tPA enzyme activities. This kit contains a fluorogenic substrate with a high reactivity and low background. tPA cleaves the substrate resulting in release of AMC (7-amino-4-methylcoumarin) fluorophore. Fluorescence can be monitored at excitation /emission= 354/442 nm. This kit can be used for high throughput screening of tPA inducers and inhibitors.

Specifications

Packaging
Kits components
  • Component A: tPA substrate, Ex/Em=354/442 nm upon substrate cleavage: 4 mM, 50 µL Component B: AMC, fluorescence reference standard, Ex/Em=354/442 nm: 4 mM, 10 µL Component C: 2X Assay Buffer: 10 mL Component D: Purified tPA enzyme: 25 Units/µL, 40 µL Component E: tPA Inhibitor: 10 mM, 100 µL
Chemistry
UniProt number
  • P00750
Properties
Absorbance (nm)
  • 354
Emission (nm)
  • 442
Storage & stability
Storage Conditions
  • Store all kit components, except Component D, at -20°C. Store Component D at -80°C. Aliquot as needed to avoid multiple freeze-thaw cycles. Protect Components A and B from light and moisture. Components C can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Reactive Center Loop (RCL) Peptides Derived from Serpins Display Independent Coagulation and Immune Modulating Activities.

J Biol Chem . 2015 Nov 30 ; 291(6) 2874 | DOI : 10.1074/jbc.M115.704841

  • S. Ambadapadi
  • et al

Exogenous t-PA administration increases hippocampal mature BDNF levels. plasmin- or NMDA-dependent mechanism?

PLoS One . 2014 Mar 26 ; 9(3) e92416 | DOI : 10.1371/journal.pone.0092416

  • M. Rodier
  • et al