Myelin oligodendrocyte glycoprotein (MOG) is a member of the immunoglobulin superfamily and is expressed exclusively in the central nervous system. Human MOG (1-125) is able to induce autoantibody production and relapsing-remitting neurological disease causing extensive plaque-like demyelination. Autoantibody response to human MOG (1-125) has been observed in induced experimental autoimmune encephalomyelitis (EAE) in DA and Lewis rats, C57/BL6 and SJL mice, and common marmoset. However, the exact pathological role and action of anti-human MOG (1-125) autoantibody is not known and is currently under vigorous investigation. This kit is optimized to detect anti-human MOG (1-125) IgG in human samples. Wells are pre-coated with recombinant human MOG (1-125) protein and pre-blocked with proprietary solution. The amount of anti- human MOG IgG in serum or plasma is quantified using ELISA. Human anti-Human MOG (1-125)standard is included. Ample materials and reagents are provided to perform 96 assays.
Component A: Human-MOG (1-125) coated and blocked 8-well strips: 12 strips
Component B: Human anti-Human MOG (1-125) IgG standard: 500 µl (100ng/ml) x 3
Component C: 1X Sample Dilution Buffer: 30 ml
Component D: 10X Wash Buffer: 50 ml
Component E: TMB color substrate solution 10 ml
Component F: Stop Solution: 10 ml
Component G: Secondary antibody, Goat anti-Human IgG-HRP: 30 µl
Storage & stability
Store all other components at 4°C for up to 6 months. Store component B at -20°C in small aliquots. Protect components E and G from light.