Mouse MOG recombinant protein (1-125) [Cat# AS-55150] is able to induces autoantibody production and relapsing-remitting neurological disease causing extensive plaque-like demyelination. Autoantibody response to mouse MOG (1-125) has been observed in induced experimental autoimmune encephalomyelitis (EAE) in C57/BL6, 129/Sv, B6, and SJL mice. The SensoLyte® Anti-Mouse MOG (1-125) IgG Quantitative ELISA Kit provides a convenient quantitative assay for anti-mouse MOG (1-125) autoantibody. This colorimetric assay is useful to researchers for determining the amount of anti-mouse MOG (1-125) antibody present, and can help provide information on the role it plays in the development and treatment of EAE, an animal model for multiple sclerosis pathogenesis.
Component A: Mouse MOG (1-125) coated and BSA blocked 8-well strips: 12 x 8 strips
Component B: Anti-mouse MOG (1-125) IgG standard: 100 µl (20 µg/ml)
Component C: 1X Sample Dilution Buffer: 30 ml
Component D: 10X Wash Buffer: 50 ml
Component E: TMB color substrate solution: 10 ml
Component F: Stop Solution: 10 ml
Component G: Secondary antibody, Goat anti-Mouse IgG-HRP: 30 µl
Storage & stability
Store all other components at 4°C for up to 6 months. Store component B at -20°C in small aliquots. Protect components E and G from light.