Assay-kits

SensoLyte® Green Elastase Assay Kit Fluorimetric - 1 kit

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  • Cat.Number : AS-72178
  • Availability :
    In stock
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Elastases catalyze the hydrolysis of elastin, the chief constituent of elastic fibers that together with collagen determines the mechanical properties of connective tissue. Elastases have many other natural substrates these include proteoglycans, collagens and fibronectin. Implicated in many diseases such as pulmonary emphysema, cystic fibrosis, infections, inflammation and atherosclerosis, elastases are considered important drug targets.
The SensoLyte® Green Elastase Assay Kit uses natural substrate elastin labeled with the 5-FAM fluorophore and the QXL™ 520 quencher. Proteolytic cleavage of labeled elastin yields brightly green fluorescence, which can be continuously monitored at excitation/emission= 488 nm/520 nm. Increase in fluorescence intensity is directly proportional to elastase activity. This kit does not require any separation steps and can be used to continuously measure the kinetics of elastase activity.

Specifications

Packaging
Kits components
  • Component A: 5-FAM/QXL™ 520 labeled elastin, Ex/Em=490/520 nm upon cleavage: 250 µL Component B: Elastase, porcine pancreas: 0.4 mg/mL, 100 µL (5 vials) Component C: 2X Assay Buffer: 30 mL Component D: Elastase inhibitor (MeOSuc-Ala-Ala-Pro-Val-CMK): 10 mM, 50 µL
Chemistry
UniProt number
  • P08246
Properties
Absorbance (nm)
  • 488
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C. Protect Component A from light and moisture. Component C can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

The voltage-gated proton channel Hv1/VSOP inhibits neutrophil granule release

J Leukoc Biol. . 2015 May 19 ; 99(1) 7 | DOI : 10.1189/jlb.3HI0814-393R

  • Y. Okochi
  • et al