Assay-kits

SensoLyte® pNPP Alkaline Phosphatase Assay Kit Colorimetric - 1 kit

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  • Cat.Number : AS-72146
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The change of alkaline phosphatase activity is involved in a variety of physiological and pathological events, such as bone development, bone-related diseases, gestation-related diseases and inflammatory bowel diseases. Alkaline phosphatase is also a popular enzyme conjugated with secondary antibody for ELISA. p-Nitrophenyl phosphate (pNPP) is proven to be an effective chromogenic substrate for alkaline phosphate.
This SensoLyte® kit can be used to detect alkaline phosphatase activity in biological samples and alkaline phosphatase-based ELISA. The kit contains: pNPP chromogenic liquid substrate (absorption maximum = 405 nm upon dephosphorylation), Assay buffer, Stop solution, Alkaline phosphatase standard, “mix and read” assay protocol that is compatible with high throughput screening.

Specifications

Packaging
Kits components
  • Component A: pNPP, colorimetric alkaline phosphatase substrate: 25 mL Component B: 10X Assay buffer: 50 mL Component C: Stop solution: 25 mL Component D: Triton-X-100: 500 µL Component E: Alkaline Phosphatase Standard, Calf Intestine: 10 µg/mL, 50 µL
Properties
Absorbance (nm)
  • 405
Storage & stability
Storage Conditions
  • Store all components at 4°C. Keep Component A away from light.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 0.2 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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