Assay-kits

SensoLyte® Luminescent Alkaline Phosphatase Assay Kit Luminometric - 1 kit

345,00
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  • Cat.Number : AS-72122
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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Alkaline phosphatase catalyzes the dephosphorylation of biomolecules in an alkaline environment and plays a key role in cell signal transduction. Besides involvement in many physiological and pathological processes alkaline phosphatase serves as a reporter for gene expression and for ELISA. SensoLyte® Luminescent Alkaline Phosphatase Assay Kit provides highly sensitive chemiluminescent substrate to quantify alkaline phosphatase activity in solutions, in cell extracts, in live cells. The kit contains: chemiluminescent substrate, positive control (calf intestine alkaline phosphatase), buffer, stop solution, an optimized ‘mix and read’ assay protocol that is compatible with HTS liquid handling instruments.

Specifications

Packaging
Kits components
  • Component A: Chemiluminescent substrate: 25 mL Component B: 10X Assay buffer: 50 mL Component C: Alkaline Phosphatase Standard, Calf Intestine: 10 µg/mL, 50 µL Component D: Triton X-100: 500 µL Component E: Stop Solution: 30 mL
Storage & stability
Storage Conditions
  • Store the kit components at 4°C. Protect Component A from light.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 1 pg/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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Citations

Formation of Human Colonic Crypt Array by Application of Chemical Gradients Across a Shaped Epithelial Monolayer.

Cmgh . 2017 Nov 03 ; 5(2) 113 | DOI : 10.1016/j.jcmgh.2017.10.007

  • Y. Yang
  • et al