Assay-kits

SensoLyte® Green PIN1 Activity Assay Kit Fluorimetric - 1 kit

509.00
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  • Cat.Number : AS-72240
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Peptidyl-prolyl isomerase (PPIase) Pin1 catalyzes cis/trans isomerization of the phospho- Serine/Threonine-Proline peptide bonds. Pin1 consists of short N-terminal protein-protein interaction domain that allows enzyme to bind phosphoproteins and longer C-terminal izomerase domain. Pin1 has many biological substrates, plays critical role in cell-cycle regulation, and up-regulated in many human cancers. Recently, Pin1 was linked to the Alzheimer’s disease pathogenesis. Pin1 can bind phosphorylated Thr-212/231 residues of Tau protein and increase its deposphorylation that may prevent formation of Tau neurofibrilary tangles.
The SensoLyte® Green Pin1 Assay Kit uses fluorogenic substrate that was pretreated to convert into cis isoform. Pin1 changes substrate into trans conformation that is readily cleaved to generate fluorescent signal which can be monitored at Ex/Em=490/520nm. Increase in fluorescence intensity is directly proportional to the Pin1 activity.

Specifications

Packaging
Kits components
  • Component A: Pin1 Green Substrate: 0.1 mM , 60 µL Component B: Pin1, Human Recombinant: 1 mg/ml, 10 µL Component C: Pin1 Developer, 100X: 50 µL, 4 vials Component D: Assay Buffer: 30 mL Component E Pin1 Inhibitor 50mM in DMSO, 25 µL
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component B at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect component A from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Codes
Code Nacres
  • NA.32

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