Assay-kits

SensoLyte® Green Protease Assay Kit Fluorimetric - 1 kit

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  • Cat.Number : AS-71124
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The SensoLyte® Green Protease Assay Kit is widely used for detection of generic protease activities. The kit uses a casein derivative that is heavily labeled with a rhodamine derivative, resulting in almost total quenching of the conjugate's fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity (Ex/Em=488/520 nm). The SensoLyte® protease assay kits do not require any separation steps and can be used to continuously measure the kinetics of a variety of exopeptidases and endopeptidases.

Specifications

Packaging
Kits components
  • Component A: Protease substrate: HiLyte Fluor™ 488-labeled casein, Ex/Em=488 nm/520 nm upon cleavage: 280 µL Component B: Trypsin 5 U/µL, 100 µL Component C: 2X Assay buffer: 30 mL
Properties
Absorbance (nm)
  • 488
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store Components A and B at -20°C. Protect Component A from light and moisture. Component C can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Antioxidant and trypsin inhibition activity of dioscorin influenced by yam cultivars and eluent pH

ifst . 2012 Apr 19 ; 47(7) 1384 | DOI : https://doi.org/10.1111/j.1365-2621.2012.02984.x

  • W-T. Kao
  • et al

Synthesis of dammarane-type triterpene derivatives and their ability to inhibit HIV and HCV proteases

Bioorg Med Chem . 2009 Mar 14 ; 17(8) 3003 | DOI : 10.1016/j.bmc.2009.03.019

  • Y. Wei
  • et al

A plant pathogen virulence factor inhibits the eukaryotic proteasome by a novel mechanism

Nature . 2008 Apr 10 ; 452(7188) 755 | DOI : 10.1038/nature06782

  • M. Groll
  • et al

Inhibitory effects of antrodins A–E from Antrodia cinnamomea and their metabolites on hepatitis C virus protease

Phytother Res . 2009 Apr 01 ; 23(4) 582 | DOI : 10.1002/ptr.2657

  • D.T. Phuong
  • et al