Assay-kits

SensoLyte® Red Protease Assay Kit Fluorimetric - 1 kit

251,00
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  • Cat.Number : AS-71140
  • Availability :
    In stock
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The SensoLyte® Red Protease Assay Kit is widely used for continuous detection of generic protease activities. The kit uses a casein derivative that is heavily labeled with a rhodamine derivative, resulting in almost total quenching of the conjugate's fluorescence. Compared to the SensoLyte® Green Protease Assay Kit, this kit contains a red fluorescent casein derivative. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly red fluorescent dye-labeled peptides (Ex/Em=546/575 nm). The increase in fluorescence intensity is directly proportional to protease activity. The SensoLyte® protease assay kits do not require any separation steps and can be used to continuously measure the kinetics of a variety of exopeptidases and endopeptidases. The kit includes: - Fluorescently labeled casein with high ratio of dye/protein - Trypsin (as positive control) - Assay buffer - A detailed protocol

Specifications

Packaging
Kits components
  • Component A: Protease substrate: 5(6)-TAMRA -labeled casein, Ex/Em=546 nm/575 nm upon cleavage: 280 µL Component B: Trypsin: 5 U/µL, 100 µL Component C: 2X Assay buffer: 30 mL
Properties
Absorbance (nm)
  • 546
Emission (nm)
  • 575
Storage & stability
Storage Conditions
  • Store Components A and B at -20°C. Protect Component A from light and moisture. Component C can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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