The SensoLyte® Plus 520 MMP-1 Assay Kit is designed for specifically detecting MMP-1 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-1 monoclonal antibody is used in combination with an MMP fluorogenic substrate, 5-FAM/QXL™520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-1-induced cleavage of the FRET substrate.
Component A: Microplate coated with monoclonal anti human MMP-1 antibody: 12 X 8 black strips
Component B: MMP-1 standard, recombinant human proMMP-1: 10 µg/mL, 10 µL
Component C: MMP dilution buffer: 10 mL
Component D: 10 X Wash buffer: 50 mL
Component E: APMA, 4-aminophenylmercuric acetate (Caution: Contains organic mercury. Dispose according to regulations): 100 mM, 150 µL Component F: MMP-1 substrate 5-FAM/QXL™520 FRET peptide Ex/Em=490 nm/520 nm upon cleavage: 50 µL
Component G: Assay
Storage & stability
Store all kit components, except Component B, at -20°C. Store Component B at -80°C. For convenience, Components D, G, H, I can be stored at RT.