Image of a kit SensoLyte Plus 520 MMP-9 Assay Kit Fluorimetric and Enhanced Selectivity
Assay-kits

SensoLyte® Plus 520 MMP-9 Assay Kit Fluorimetric and Enhanced Selectivity - 1 kit

€663.00
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  • Cat.Number : AS-72017
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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The SensoLyte® Plus 520 MMP-9 Assay Kit is designed for specifically detecting MMP-9 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-9 monoclonal antibody is used in combination with a MMP fluorogenic substrate, 5-FAM/QXL™520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-9-induced cleavage of the FRET substrate.

Specifications

Packaging
Kits components
  • Component A: Microplate coated with monoclonal anti human MMP-9 antibody: 12 X 8 black strips Component B: MMP-9 standard, recombinant human proMMP-9: 10 µg/mL, 10 µL Component C: MMP dilution buffer: 5 mL Component D: 10 X Wash buffer: 50 mL Component E: APMA, 4-aminophenylmercuric acetate (Caution: Contain organic mercury. Dispose it according to your local regulations): 100 mM, 150 µL Component F: MMP-9 substrate, 5-FAM/QXL™520 FRET peptide Ex/Em=490 nm/520 nm upon cleavage: 50 µL Component G: Assay buffe:r 50 mL Component H: Stop Solution: 10 mL Component I: Adhesive cover strip: 3 sheets
Chemistry
UniProt number
  • P14780
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component B at -80 °C. Store all other components at -20 °C. Components D, G, H and I can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 3 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Codes
Code Nacres
  • NA.32

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Citations

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  • et al

Inhibition of MMP-3 or -9 suppresses lipopolysaccharide-induced expression of proinflammatory cytokines and iNOS in microglia

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  • M.S. Woo
  • et al

The functional interplay between EGFR overexpression, hTERT activation, and p53 mutation in esophageal epithelial cells with activation of stromal fibroblasts induces tumor development, invasion, and differentiation

Genes Dev. . 2007 Nov 01 ; 21 2788 | DOI : 10.1101/gad.1544507

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HMGA1 Is a Determinant of Cellular Invasiveness and In vivo Metastatic Potential in Pancreatic Adenocarcinoma

Cancer Res . 2006 Dec 05 ; 66 11613 | DOI : 10.1158/0008-5472.CAN-06-1460

  • S-S. Liau
  • et al

Decorin antagonizes the angiogenic network: concurrent inhibition of Met, hypoxia inducible factor 1α, vascular endothelial growth factor A, and induction of thrombospondin-1 and TIMP3

J Biol Chem . 2012 Feb 17 ; 287 5492 | DOI : 10.1074/jbc.M111.283499

  • T. Neill
  • et al

Proteolytic activity in wound fluids and tissues derived from chronic venous leg ulcers

Wound Repair Regen.  . 2009 Nov 01 ; 17 832 | DOI : 10.1111/j.1524-475X.2009.00547.x

  • A.N. Moor
  • et al