The SensoLyte® Plus 520 MMP-9 Assay Kit is designed for specifically detecting MMP-9 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-9 monoclonal antibody is used in combination with a MMP fluorogenic substrate, 5-FAM/QXL™520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-9-induced cleavage of the FRET substrate.
Component A: Microplate coated with monoclonal anti human MMP-9 antibody: 12 X 8 black strips
Component B: MMP-9 standard, recombinant human proMMP-9: 10 µg/mL, 10 µL
Component C: MMP dilution buffer: 5 mL
Component D: 10 X Wash buffer: 50 mL
Component E: APMA, 4-aminophenylmercuric acetate (Caution: Contain organic mercury. Dispose it according to your local regulations): 100 mM, 150 µL
Component F: MMP-9 substrate, 5-FAM/QXL™520 FRET peptide Ex/Em=490 nm/520 nm upon cleavage: 50 µL
Storage & stability
Store all kit components, except Component B, at -20°C. Store Component B at -80°C. For convenience, Components D, G, H, I can be stored at RT.
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