Assay-kits

SensoLyte® Rh110 Factor Xa Assay Kit Fluorimetric - 1 kit

422,00
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  • Cat.Number : AS-72207
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    In stock
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Factor Xa (FXa) is a serine endopeptidase composed of two disulfide-linked subunits. FXa leads to blood clot formation by converting prothrombin to thrombin through the prothrombinase complex. FXa is generated from zymogen Factor X via the intrinsic and extrinsic pathways and is the rate-limiting step in the propagation of thrombin generation. In the presence of Ca2+ ions, FXa forms prothrombinase with factor Va on the phospholipid membrane of the activated platelets. FXa has emerged as an attractive target for drug discovery for thromboembolic diseases.
The SensoLyte® Rh110 Factor Xa Assay Kit provides a convenient assay for screening of FXa inhibitors or continuous assay of enzyme activity using a fluorogenic substrate. Upon FXa protease cleavage, this substrate generates the Rh110 (rhodamine 110) fluorophore which has a bright green fluorescence that can be detected at excitation/emission=490 nm/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from other reaction components.

Specifications

Packaging
Kits components
  • Component A: Rh110 Factor Xa substrate, Ex/Em=490 nm/520 nm upon cleavage: 0.4 mM, 50 µL Component B: Rh110, fluorescence reference standard, Ex/Em=490 nm/520 nm: 0.4 mM, 10 µL Component C: Purified Bovine Factor Xa: 5 ng/µL, 20 µL Component D: 2X Assay Buffer: 25 mL Component E: Factor Xa Inhibitor: 1 mM, 20 µL
Chemistry
UniProt number
  • P00742
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except for Component C. Store Component C at -80°C. Protect Components A and B from light and moisture. Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 0.31 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Novel FXa Inhibitor Identification through Integration of Ligand- and Structure-Based Approaches

Molecules . 2017 Sep 22 ; 22 E1588 | DOI : 10.3390/molecules22101588

  • F.C. Lagos
  • et al