Labeling-detection

SMCC Activated R-PE (R-Phycoerythrin) - 1 mg

139,00
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  • Cat.Number : AS-72110
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Quantity

R-Phycoerythrin (R-PE), a fluorescent protein from phycobiliprotein family, is isolated from red algae. SMCC Activated R-PE is chemically modified with SMCC. SMCC reacts with the primary amine on R-PE and introduces maleimide groups to R-PE. These maleimide groups easily react with thiol groups of target protein without the need for any additional activation, resulting in convenient conjugation of R-PE with proteins. R-PE’s primary absorption peak is at 565 nm with secondary peaks at 496 and 545 nm. The broad excitation spectrum provides the advantage for multi-color immunofluorescent staining or cell sorting. R-PE and the closely related BPE are the most intensely fluorescent phycobiliproteins with orange fluorescence. They are significantly brighter and more photostable than conventional organic fluorophores. R-PE conjugates are widely used in applications such as flow cytometry, live cell staining, and immunofluorescent staining.

Specifications

Properties
Absorbance (nm)
  • 565
Emission (nm)
  • 578
Quantity & Purity
Concentration
  • 1 mg/ml
Storage & stability
Form
  • In solution
Storage Buffer
  • MES buffer, pH 6.0 with EDTA and preservative
Storage Conditions
  • The SMCC activated protein is stable for ~12 months at 4°C if kept from light. Do NOT freeze
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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