PCR & qPCR

Takyon™ Low ROX Probe 2X MasterMix dTTP blue

364.00
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  • Cat.Number : UF-LPMT-B0701
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Select specification

Size

Quantity

Takyon™ qPCR MasterMixes Blue for Probe assays containing Low ROX passive reference are available in different formats from 1.5 ml to 10 x 7.5 ml. Takyon™ Kits deliver FAST, SENSITIVE and ACCURATE results on the following FAST & regular Cycling conditions

On the Mx3000P®, Mx3005® and Mx4000®, both MasterMixes with and without ROX passive reference can be used depending on your needs. If you do not perform ROX normalisation on your results, we advise you to use the Takyon Kits for Probe Assay - No ROX which have been validated on these thermocyclers.
For carry-over prevention, combine any dTTP Mastermix with the new dUTP/UNG additive (RT-UTPUNG-020).
To convert any dTTP 2X Mastermix into a One-step RT-qPCR Kit, please use the Takyon™ One-step kit converter for 150 rxns: UF-RTAD-D0101, for 750 rxns: UF-RTAD-D0701

Avoid repeated freeze-thaw cycles.

Specifications

Packaging
Kits components
  • Sample and 150 reactions kit contain: One tube (1.5 mL) of 2X MasterMix (red cap) containing Takyon™ DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, Rox Passive reference, Stabilizers, Inert blue dye. 1 x 1.5 ml 50 mM MgCl2 (clear cap; optional use) 750 reactions kit contains each: One bottle (7.5 mL) of 2X MasterMix (red cap) containing Takyon™ DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, Rox Passive reference, Stabilizers, Inert blue dye. 1 x 1.5 ml 50 mM MgCl2 (clear cap; optional use)
Properties
Format
Color
Quantity & Purity
Concentration
  • 2 X
Storage & stability
Form
  • In solution
Storage Conditions
  • For long term storage the Takyon™ qPCR MasterMixes should be stored between -15 °C to -25 °C in a constant temperature freezer. When stored under these conditions the reagents are stable during 24 months. For short term storage the Takyon™ qPCR MasterMixes can be stored at 4 °C for 6 months.
Activity
qPCR compatible thermocyclers
Application
Template type
  • cDNA/DNA
Volume activity
  • 20 µL
Detection Method
Usage
  • Research use
Codes
Code Nacres
  • NA.55

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Citations

Seed sequence polymorphism rs2168518 and allele-specific target gene regulation of hsa-miR-4513

Human Molecular Genetics . 2021 Oct 04 ; 1-13 | DOI : https://doi.org/10.1093/hmg/ddab292

  • Christina Kiel
  • et al

PTEN regulates adipose progenitor cell growth, differentiation, and replicative aging

ScienceDirect . 2021 Jul 14 ; 297(2) 100968 | DOI : https://doi.org/10.1016/j.jbc.2021.100968

  • Anna S. Kirstein
  • et al

Gut-brain communication by distinct sensory neurons differently controls feeding and glucose metabolism

ScienceDirect . 2021 May 26 ; 7 1466-1482 | DOI : https://doi.org/10.1016/j.cmet.2021.05.002

  • Diba Borgmann
  • et al

Development and optimization of a TaqMan assay for Nosema bombycis, causative agent of pébrine disease in Bombyx mori silkworm, based on the β-tubulin gene

ScienceDirect . 2021 May 13 ; (2021) 106238 | DOI : https://doi.org/10.1016/j.mimet.2021.106238

  • Anupama Jagadish
  • et al

References

Optimized methods for detecting Salmonella Typhi in the environment using validated field sampling, culture and confirmatory molecular approaches

sfam . 2021 Jul 29 ; 2021;00 1-15 | DOI : https://doi.org/10.1111/jam.15237

  • Jonathan Rigby
  • et al