Image of a kit Takyon No Rox Probe Mmx dTTP blue 1250RXN
PCR & qPCR

Takyon® No ROX Probe 2X MasterMix Blue dTTP

€393.00
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  • Cat.Number : UF-NPMT-B0701
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Takyon® qPCR Kits for Probe assays containing no ROX passive reference are available in different MasterMix formats from 1.5 ml to 10 x 7.5 ml. Takyon® Kits deliver FAST, SENSITIVE and ACCURATE results in FAST & Regular Cycling conditions

For carry-over prevention, combine any dTTP Mastermix with the new dUTP/UNG additive (RT-UTPUNG-020)

To convert any dTTP 2X Mastermix into a One-step RT-qPCR Kit, please use the Takyon® One-step kit converter (UF-RTAD-D0101).

Avoid repeated freeze-thaw cycles.

Specifications

Packaging
Kits components
  • Sample and 150 reactions kit contain: One tube (1.5 mL) of 2X MasterMix (black cap) containing Takyon® DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, Inert blue dye, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use) 750 reactions kit contains each: One bottle (7.5 mL) of 2X MasterMix (black cap) containing Takyon® DNA polymerase, MgCl2 (5.5mM final concentration), dNTPs, Inert blue dye, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use)
Properties
Format
Color
Quantity & Purity
Concentration
  • 2 X
Storage & stability
Form
  • In solution
Storage Conditions
  • For long term storage the Takyon® No Rox Probe MasterMix dTTP blue should be stored at a temperature between -15 °C and -25°C in a constant temperature freezer. When stored under these conditions, the components are stable for 24 months. For short term storage the Takyon® No Rox Probe MasterMix dTTP blue can be stored at 4 °C for 6 months.
Activity
qPCR compatible thermocyclers
Application
Template type
  • cDNA/DNA
Volume activity
  • 20 µL
Detection Method
Usage
  • Research use
Codes
Code Nacres
  • NA.55

Downloads

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Citations

The AhR ligand 2, 2′-aminophenyl indole (2AI) regulates microglia homeostasis and reduces pro-inflammatory signaling

ScienceDirect . 2021 Nov 19 ; 579 15-21 | DOI : https://doi.org/10.1016/j.bbrc.2021.09.054

  • Amir Saeed Khan
  • et al

Comparison of Three In-House Real PCR Assays Targeting Kinetoplast DNA, the Small Subunit Ribosomal RNA Gene and the Glucose-6-Phosphate Isomerase Gene for the Detection of Leishmania spp. in Human Serum

Pathogens . 2021 Jun 30 ; 2021, 10(7), 826 1-11 | DOI : https://doi.org/10.3390/pathogens10070826

  • Konstantin Tanida
  • et al

Temporal dynamics of the Hyalomma marginatum-borne pathogens in southern France

. 2024 Sep 07 ; | DOI : https://doi.org/10.1016/j.crpvbd.2024.100213

  • Charlotte Joly-Kukla
  • et al

Expression of fungal and host markers in models of dermatophytosis on mice and human epidermis

Journal of Investigative Dermatology . 2024 Aug 30 ; | DOI : https://doi.org/10.1016/j.jid.2024.08.010

  • Emilie Faway