PCR & qPCR

Takyon™ No ROX SYBR 2X MasterMix blue dTTP

364.00
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  • Cat.Number : UF-NSMT-B0701
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Select specification

Size

Quantity

Takyon™ qPCR Kits for SYBR® assays containing no ROX passive reference are available in different MasterMixes formats from 1.5 ml to 10 x 7.5 ml. Takyon Kits deliver FAST, SENSITIVE and ACCURATE resultsin FAST & regular thermocyclers

For carry-over prevention, combine any dTTP MasterMix with the new dUTP/UNG additive for 150 rxns: RT-UTPUNG-005, for 750 rxns: RT-UTPUNG-020
To convert any dTTP 2X Mastermix into a One-step RT-qPCR Kit, please use the Takyon™ One-step kit converter for 150 rxns: UF-RTAD-D0101, for 750 rxns: UF-RTAD-D0701

Avoid repeated freeze-thaw cycles.

Specifications

Packaging
Kits components
  • Sample and 150 reactions kit contain: One tube (1.5 mL) of 2X MasterMix (black cap - amber tube) containing Takyon™ DNA polymerase, MgCl2 (2.5mM final concentration), SYBR Green®, dNTPs, Inert blue dye, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use) 750 reactions kit contains each: One bottle (7.5 mL) of 2X MasterMix (black cap - amber vial) containing Takyon™ DNA polymerase, MgCl2 (2.5mM final concentration), SYBR Green®, dNTPs, Inert blue dye, Stabilizers. 1.5 mL of 50 mM MgCl2 (clear cap; optional use)
Properties
Format
Color
Quantity & Purity
Concentration
  • 2 X
Storage & stability
Form
  • In solution
Storage Conditions
  • For long term storage the Takyon™ No Rox SYBR® MasterMix dTTP blue should be stored at a temperature between -15°C and -25°C in a constant temperature freezer. When stored under these conditions, the components are stable for 12 months. For short term storage the Takyon™ No Rox SYBR® MasterMix dTTP blue can be stored at 4°C for 6 months. Product must be stored in the dark.
Activity
qPCR compatible thermocyclers
Application
Template type
  • cDNA/DNA
Volume activity
  • 20 µL
Detection Method
Usage
  • Research use
Codes
Code Nacres
  • NA.55

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Citations

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Comparative genomics provides structural and functional insights into Bacteroides RNA biology

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T helper cell-licensed mast cells promote inflammatory Th17 cells

ResearchGate . 2021 Jul 28 ; | DOI : https://doi.org/10.1101/2021.07.28.454103

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Coxsackievirus B4 Transplacental Infection Severely Disturbs Central Tolerogenic Mechanisms in the Fetal Thymus

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Resolvin D1 reduces expression and secretion of cytokines and monocyte adhesion triggered by Angiotensin II, in rat cardiac fibroblasts

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Evidence of dysregulated iron homeostasis in newly diagnosed diabetics, but not in pre-diabetics

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Targeted PI3K/AKT-hyperactivation induces cell death in chronic lymphocytic leukemia

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Cystine–glutamate antiporter deletion accelerates motor recovery and improves histological outcomes following spinal cord injury in mice

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Environmental Conditions Modulate the Transcriptomic Response of Both Caulobacter crescentus Morphotypes to Cu Stress

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Activation of GSK3 Prevents Termination of TNF-Induced Signaling

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