Image of a Takyon No Rox SYBR Core Kit blue dTTP
PCR & qPCR

Takyon™ No Rox SYBR Core Kit blue dTTP

€442.00
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  • Cat.Number : UF-NSCT-B0201
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Takyon™ Core Kits for qPCR SYBR assays containing no ROX passive reference are available in 1250 (20 µL) RXNs format. Takyon™ Kits deliver FAST, SENSITIVE and ACCURATE results in FAST & Regular Cycling conditions. Core kits contain components in separated vials to allow maximum flexibility in assay formulation as the concentration of the different components can be optimized to get the best possible results.

For carry-over prevention, use dUTP/UNG additive included in the kits

Avoid repeated freeze-thaw cycles.

Specifications

Packaging
Kits components
  • Each kit contains: 2 Tubes (1.5 mL) of 10x Buffer (brown cap) containing KCl and Tris-HCl, Stabilizers, Inert Blue dye 2 Tubes (1.5 mL) of 50 mM MgCl2 (clear cap; optional use) 1 Tube (1.25 mL) of 5 mM dNTPs (green cap) containing a blend of dATP, dCTP, dGTP and dTTP 1 Tube (125 µL) of Takyon™ enzyme (5 U/µL) (yellow cap) 1 Tube (1 mL) of DMSO 1 Tube (330 µL) of dUTP and Uracyl N-glycosylase blend for carryover prevention 1 tube of SYBR® Green I (amber tube)
Properties
Format
Color
Quantity & Purity
Concentration
  • Separated components
Storage & stability
Form
  • In solution
Storage Conditions
  • For long term storage the Takyon™ No Rox SYBR® Core Kit dTTP blue should be stored at a temperature between -15 °C and -25°C in a constant temperature freezer. When stored under these conditions, the components are stable for 24 months. For short term storage the Takyon™ No Rox SYBR® Core Kit dTTP blue can be stored at 4°C for 6 months. The SYBR® Green I is light sensitive and should be kept away from light as much as possible.
Activity
qPCR compatible thermocyclers
Application
Template type
  • cDNA/DNA
Volume activity
  • 20 µL
Detection Method
Usage
  • Research use
Codes
Code Nacres
  • NA.55

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Citations

Embryonic thermal manipulation impacts the postnatal transcriptome response of heat-challenged Japanese quails

BMC Genomics . 2021 Jun 30 ; (2021) 22:488 1-13 | DOI : https://doi.org/10.1186/s12864-021-07832-7

  • Anaïs Vitorino Carvalho
  • et al