Takyon™ Core Kits for qPCR SYBR assays containing no ROX passive reference are available in 1250 (20 µL) RXNs format. Takyon™ Kits deliver FAST, SENSITIVE and ACCURATE results in FAST & Regular Cycling conditions. Core kits contain components in separated vials to allow maximum flexibility in assay formulation as the concentration of the different components can be optimized to get the best possible results.
For carry-over prevention, use dUTP/UNG additive included in the kits
Avoid repeated freeze-thaw cycles.
Each kit contains: 2 Tubes (1.5 mL) of 10x Buffer (brown cap) containing KCl and Tris-HCl, Stabilizers, Inert Blue dye 2 Tubes (1.5 mL) of 50 mM MgCl2 (clear cap; optional use) 1 Tube (1.25 mL) of 5 mM dNTPs (green cap) containing a blend of dATP, dCTP, dGTP and dTTP 1 Tube (125 µL) of Takyon™ enzyme (5 U/µL) (yellow cap) 1 Tube (1 mL) of DMSO 1 Tube (330 µL) of dUTP and Uracyl N-glycosylase blend for carryover prevention 1 tube of SYBR® Green I (amber tube)
For long term storage the Takyon™ No Rox SYBR® Core Kit dTTP blue should be stored at a temperature between -15 °C and -25°C in a constant temperature freezer. When stored under these conditions, the components are stable for 24 months. For short term storage the Takyon™ No Rox SYBR® Core Kit dTTP blue can be stored at 4°C for 6 months. The SYBR® Green I is light sensitive and should be kept away from light as much as possible.