At Eurogentec, monoclonal antibodies are produced exclusively in vitro from hybridoma , in accordance with the Belgian animal regulations and the 2010-63-EU directive for animal welfare.
Our facilities are equipped to produce from mg to gram mAb scale.
For requests of 200mg and higher, a test study is systematically performed to evaluate the clone productivity.
The Best production scheme
Based on the requested mAb amount and the clone productivity, we select the best production scheme using flasks, roller bottles or bioreactors.
We adapt the conditions for mouse, human, or any other species monoclonal antibody production.
mAb production scale
Depending on the mAb amount requested and the hybridoma production yield,
we select for you the most adapted production system which will guaranty the best production level.
For large-scale production batches (>200 mg), a test run is always performed, to assess the clone productivity.
The cost for this test run is deduced from the subsequent production run to which the defined parameters are applied.
A large range of customization is available to tailor the program to your specific needs.
By mixed-mode chromatography
Affinity chromatography (protein A,G,L), ion exchange, gel filtration chromatography, IgM purification
Solutions for DNA and endotoxins removal
Concentration / Diafiltration
Following your specifications
Conjugation and freeze-drying services
Purity, Quantity, Integrity, Sterility,…
Method validation (ELISA, FACS, IHC,…)
Binding, cytotoxicity, antibody-dependent cell-mediated cytotoxicity (ADCC), CDC, residual endotoxins, in vivo testing…