Speedy 28-Day is the reference program to produce custom polyclonal antibodies at Eurogentec.
This proprietary program uses a non-Freund adjuvant that stimulates the immune response and
gives mostly IgG’s, despite the short immunization time.
You receive one shipment:
The pre-immune (negative control), intermediate bleed, and final bleed (unless you decide to adapt the program) are delivered at the end of the program.
Speedy 28-Day is recommended for :
A strong and quick response
An anti-PTM program
A quick access to purified and ready to use pAb
Our classical programs provide high titer antibodies with excellent affinity.
Immunization lasts from 65 to 90 days depending on the host and the immune response. We can monitor the immune response after two months using the second intermediate bleed, and deliver the pre-immune and second intermediate bleeds to the customer for on site testing. This allows determining if the final bleed should be maintained as initially scheduled, or if a program adaptation is necessary.
You receive two shipments:
The pre-immune (negative control) and intermediate bleeds, delivered while the program is still running
The final bleed (unless you decide to adapt the program).
The classical programs are recommended when :
Repeating a pre-existing protocol already conducted with a classical program
Immunizing a host where the Speedy 28-Day program is not available
or not guaranteed (chicken, mouse, large animals,…)
Using an antigen of poor immunogenicity that may require a program prolongation
*This schedule is indicative and is subject to variations according to the program specifications
The lower the homology, the higher the immune response.
When working with highly conserved mammalian proteins, it may be useful to use chicken as the host, which produce IgY and not IgG
If a high batch size is requested, one may immunize one goat or sheep (~1L serum per animal), several rabbits in parallel (~50ml serum each), or immunize one hen and collect several eggs (4 eggs contains as much antibody as the serum of one rabbit)
If double-staining experiments are to be performed, one may recommend combining one mammal (eg rabbit or mouse) and one hen, because the anti-IgG will not cross-react onto IgY, and vice versa
Immunizing one mouse requires 15µg protein per injection, while goat immunization will require 200µg per injection
Antigen quantity and bleed volume comparison
|Min. antigen quantity / injection||Bleed volumes / animal|
< 18 kDa
> 18 kDa
|Pre-immune||Small Bleed||Large bleed||Final bleed||Comment|
|Mouse||40 µg||15 µg||40-70 µl||40-70 µl||40-70 µl||300-500 µl||Good to test antigenicity|
|Guinea pig||50 µg||30 µg||1 ml||1 ml||2-3 ml||10-15 ml||For small serum volumes|
|Rat||50 µg||30 µg||± 2 ml||± 2 ml||± 2 ml||5-7 ml||For small serum volumes|
|Chicken||200 µg||100 µg||1 egg||1 egg||± 8-10 eggs||± 8-10 eggs||For mammalian antigens and large quantities of Antibodies, 4 eggs = 1 rabbit final bleed|
|Rabbit||200 µg||100 µg||2 ml||2 ml||20-25 ml||50-70 ml||For most applications|
|Goat||400 µg||200 µg||2 ml||2 ml||250 ml||1000 ml||For large batch volumes|
|Sheep||400 µg||200 µg||2 ml||2 ml||250 ml||1000 ml||For large batch volumes|
in max 5 ml
|200 µg||2 ml||2 ml||250 ml||1000 ml||Single chain antibodies and for large batch volumes|
|Horse||400 µg||200 µg||2 ml||2 ml||250 ml||1000 ml||For large batch volumes|
|Other||Contact us for recommendations|
Any program can be adapted
We can adapt to your requirements, including animal sanitary check and reporting, serum batch selection, highly documented productions, large batches supply, and long-term supply with high reproducibility, among others.
The most frequent deliverables are crude serum and purified antibodies, but we can also isolate organs (bone marrow, spleen, lymph nodes..) and cells (B-cells…).