Antibodies specialized programs
Using non-mammalian hosts like chicken allows obtaining good immune responses when the antigen is a highly conserved mammalian protein. Moreover, chicken antibodies are present at high quantities in the egg yolk, allowing a non-invasive antibody collection.
Several antigens such as recombinant proteins and peptides may be used, provided by the customer or produced by Eurogentec.
If using peptides, ‘Double X programs’, where a combination of 2 peptides targeting different protein regions are co-injected, will increase the chances of success.
|You provide the antigena (peptide, protein, complex sample)||Eurogentec designsc and synthesises one peptide||Eurogentec designs and synthesises two peptides to increase the chances of success|
|Peptide synthesis||X||1 peptide||2 peptides|
|>70% purity||>70% purity|
|10-16 aa||10-16 aa|
|15-25 mg||15-25 mg|
|Coupling to carrier||(O)||5 mg||5 mg of each peptide|
|or MAP carrier||or MAP carrier||or MAP carrier|
|Number of host||2||2||2|
|Delivery||Egg yolks||Egg yolks||Egg yolks|
|Purified antibody (O)||Purified antibody (O)||Purified antibody (O)|
|Remaining peptided||Remaining peptided|
a. See the section "How to provide your antigen"
b. The guarantee only applies for peptides when approved by Eurogentec
c. Please note that peptide design aims at conferring high antigenicity to the peptides, but doesn’t guaranty that they will lead to antibodies functional in any application. To guarantee an optimal functionality of the generated antibodies in your application, we suggest our DoubleX strategy which involves the design and co-injection of two peptides from the same antigen. This co-immunisation more closely mimics the protein than a single peptide strategy and has success rates in the 90 % in Western Blots using this technique.
d. There is no minimum amount of remaining peptide quaranteed after coupling, ELISA test and purification. If a specific quantity of unconjugated peptide is requested, it will be provided as separated aliquots for an additional fee.
X not applicable