One-Step qPCR kit
Optimized RT-qPCR reagents for Viral RNA detection
We have developed Takyon® Ultra - a unique reverse transcription qPCR MasterMix with increased multiplex capabilities. This RT-qPCR kit has been optimized for the sensitive and specific detection of viral RNA templates such as that from SARS-CoV-2, influenza and RSV. The kit has been validated on DNA targets.
20 µL RXNs package size
Advantages of our RT-qPCR Mix
Our Probe based One-Step Takyon® Ultra RT qPCR MasterMix with a broad dynamic range
enables specific, reproducible and highly sensitive detection of viral RNA including RNA from SARS-CoV-2.
Combined with the improved Euroscript II Plus, your high throughput automated assays
can be conveniently assembled at room temperature.
Takyon® Ultra is compatible with all qPCR instruments. The MasterMix can be easily adjusted to the appropriate ROX™ level in a single initial pipetting step using the included ROX vial.
Our MasterMix formulation has been optimized to ensure more specific results even in low target copy context and clean Non template Controls (NTC).
Our new reagent enables increased multiplexing capabilities allowing for co-detection in clinical samples of various pathogens such as SARS-CoV-2, influenza and RSV
The 4x MasterMix is perfectly suited for robotic platforms. The reagents are stable at 20°C for up to 24h allowing for convenient automated high-throughput assay set-up.
Specific and brilliant
Figure 1. Sensitivity and specificity of Sarbeco E gene (SARS-Cov-2 RNA viral target) detection in samples from known COVID-19 positive (patients 1 & 2) or negative (patient 3) patients.
The One-Step Takyon® Ultra Probe 4X MasterMix (yellow) shows increased sensitivity compared to Competitor T (dark blue) and Competitor B (light blue) reagents, and increased specificity compared to competitor T, under their recommended fast cycling conditions.
Sensitive and efficient
Figure 2. Linear dynamic range in 10-fold dilutions from 500 000 down to 5 copies of SARS-COV-2 RNA Control (TWIST Bioscience #MT007544.1).
One-Step Takyon® Ultra Probe 4X MasterMix (yellow) shows a better linear dynamic range than competitor T (dark blue) and better signal than competitor B (light blue) reagents. The amplifications were performed in presence of Yeast T-RNA , under the recommended fast cycling conditions. (NTC: dotted lines)
Figure 3. 10-fold linear range from 100 000 down to 10 copies of SARS-COV-2 RNA Control (TWIST Bioscience #MT007544.1) under different storage temperatures for 24h.
The One-Step Takyon® Ultra Probe 4X MasterMix maintains consistent amplification results after 24h at -20°C (dark blue, recommended storage conditions), 4°C (light blue) or 20°C (yellow) under fast cycling conditions. (NTC: dotted lines)
Quantitative RT-PCR protocol
The One-Step Takyon® Ultra Probe 4X MasterMix produces consistent and sensitive results under Fast cycling conditions.
For increased convenience, a working blend of MasterMix and Euroscript II RT Plus can be prepared and stored at -20°C for up to one month.
This one-tube solution is also stable at 20°C for up to 24h allowing for time-consuming high-throughtput assay set-up.