Dual Labeled Custom Probes
Custom qPCR and dPCR probes
As a recognized oligo synthesis company since 1985, we produce various types of custom probes including TaqMan® and Molecular Beacon probes for quantitative and digital PCR. Thanks to our vast portfolio of fluorescent dyes and non-fluorescent quenchers (NFQ), our dual labeled probes can be used in any qPCR channels from deep blue to Infrared.
Discover our custom qPCR probes
| Double Dye | MGB probes | Molecular Beacons | |
|---|---|---|---|
| Length | from 8 to 45 bases | from 8 to 30 bases | From 28 to 50 bases |
| Purification | HPLC | HPLC | HPLC |
| Quality Control | MALDI-TOF MS + UHPLC | MALDI-TOF MS + UHPLC | MALDI-TOF MS + UHPLC |
| Formats | Lyophilized in individual tubes or in solution (H2O or TE) | In solution (H2O or TE); dried or dried aliquoted in 10 nmol on request | In solution (H2O or TE); dried in individual tubes |
| Quantities | 10 nmol • 40 nmol • 200 nmol • 1000 nmol • 2.5 µmol • 5 µmol • 10 µmol • 20 µmol (Synthesis scale) |
6 – 20 – 50 nmol (Delivered quantity) |
40 nmol • 200 nmol • 1000 nmol • |
| Backbones | DNA, LNA® | DNA | DNA, LNA® |
| Order now | Order now | Order now |
Don’t miss out our offer!
For each probes configured online, receive two free primers.
How to select your qPCR and dPCR probe?
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Select the probe type
Dual labeled Probes are TaqMan® probes and are widely used because they are most efficient and offer sequence specificity SYBR® Green does not. To increase sensitivity and specificity, MGB probes are recommended. -
Select your fluorescent dye
This step is important to select the most appropriate dye according to your thermocycler. To help you in dye selection, please refer to the compatibility cycler channels versus fluorescent dyes table.
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Choose the quencher adapted to your dye
Each fluorescent dye emits at a specific maximum wavelength. The best quenching result is obtained with quenchers that absorb in the same range. To select the best dye/quencher couple, you can consult the dyes & quencher compatibility table.
Double dye probes
Double-Dye probes are labeled with a fluorescent reporter dye and a quencher at their 5’ and 3’ ends, respectively. The probes are available with classic DNA backbone, but also Locked Nucleic Acid (LNA®) for higher thermal stability, specificity and reproducibility. Various fluorescent dyes are available according to the selected quencher e.g. FAM/BHQ®-1, FAM/TAMRA, HEX/EDQ, ROX/BHQ®-2 or ATTO/BHQ® couples.
Minor Groove Binder (MGB) probes
MGB probes are dual-labeled probes incorporating a Minor Groove Binder at their 3´end. MGB Eclipse® probes ordered at Eurogentec are not limited by any license, and can be used for any applications all around the world! With our portfolio of fluorescent dyes for MGB Eclipse® probes, our offer covers all qPCR channels. It will allow you to increase the specificity, sensitivity and efficiency of your assay.
Molecular Beacons
We offer standard, wavelength-shifting and 2' O-Me RNA Molecular Beacon probes.
Our custom synthesized Molecular Beacons are available with DABCYL or BHQ® non fluorescent quenchers. Other dyes are available upon request.
Label your probes with our extensive
selection of dyes and quenchers
Discover our extensive list of dyes and quenchers for your qPCR and dPCR assays.
Check our online configurators to discover the full selection.
| Channels | Fluorescent dyes | Absorbance λmax (nm) | Emission λmax (nm) | Quenchers |
|---|---|---|---|---|
| ATTO 390 | 390 | 476 | BHQ®-1, MGB-EDQ | |
| ATTO 425 | 439 | 485 | BHQ®-1, MGB-EDQ | |
| FAM | 492 | 518 | BHQ®-1, MGB-EDQ | |
| ATTO 488 | 500 | 520 | BHQ®-1, MGB-EDQ | |
| TET | 521 | 536 | BHQ®-1, MGB-EDQ | |
| Yakima Yellow* | 530 | 549 | BHQ®-1, MGB-EDQ | |
| JOE | 529 | 555 | BHQ®-1, MGB-EDQ | |
| HEX | 535 | 556 | BHQ®-1, MGB-EDQ | |
| Dragonfly orange™ | 554 | 576 | BHQ®-2, MGB-EDQ | |
| ATTO 550 | 554 | 576 | BHQ®-2, MGB-EDQ | |
| ATTO 565 | 564 | 590 | BHQ®-2, MGB-EDQ | |
| ROX™ | 575 | 602 | BHQ®-2, MGB-EDQ | |
| Texas Red® | 597 | 616 | BHQ®-2, MGB-EDQ | |
| ATTO 590 | 593 | 622 | BHQ®-2 | |
| ATTO 647N | 646 | 664 | BHQ®-2/-3 | |
| Cy® 5 | 650 | 670 | BHQ®-2 | |
| Cy® 5.5 | 675 | 694 | BHQ®-3, QXL® 670 | |
| ATTO 700 | 700 | 716 | BHQ−3 | |
| ATTO 740 | 743 | 763 | IRQXL® |
Primer
probe ratio
Optimal qPCR result may require primers and probe titration or adjustment of the primer/probe ratio.
Fluorophore and quencher pair
With our Dyes and Quenchers Table find the most appropriate couple for your equipment.
Minimum guaranteed yield
Get an estimation of the
amount of Dual labeled probe you will receive.
At your side from research
to commercialization
We produce and deliver your nucleic acid sequences for research, diagnostic and commercial use.
From Research grade to Diagnostics
Our oligonucleotides can be used for research, pre-diagnostic, or diagnostic applications.
Our processes are certified under ISO 9001 and ISO 13485 to ensure that we meet the quality standards for your qPCR and dPCR probes. Moreover, our IVD-grade oligos are manufactured in classified clean rooms to meet the needs of your application.
We also provide a comprehensive support all along your project development. From research to commercialization, our team of experts will accompany you.
Solutions from small to large scales
With over 35 years of expertise, we produce high-quality oligonucleotides at mid to large scales, supporting your diagnostic project. Trusted by research institutions, pharmaceutical, and biotech companies worldwide, we deliver reliable solutions tailored to your specific needs.
We produce your oligos in our state-of-the-art facilities. We establish a robust process that will assure the high quality of your oligonucleotide.
IP-free dyes and quenchers offer
We can propose a large panel of IP-free dyes and quenchers to ensure a smooth transition from research to commercialization.
An IP-free portfolio for Diagnostics
The Access™ portfolio offers a comprehensive range of IP-free dye and quencher pairs for qPCR and dPCR probes, covering the entire visible spectrum from blue to infrared (IR). Designed to maximize probe performance, our dye-quencher combinations ensure high signal intensity for a precise fluorescence detection across all qPCR and dPCR assay channels. Streamline your assay development with IP-free dyes and quenchers, ensuring a smooth transition from research to commercialization without unexpected licensing barriers.
IP-free ATTO dyes for all qPCR channels
ATTO dyes are highly sensitive and bright dyes. They are known for their stability and ensure reliable results for qPCR and dPCR assays. We offer an extensive range of IP-free ATTO dyes, ideal for any research and commercial applications including human diagnostics, veterinary use and agro-food testing.
Our ATTO dyes are sourced directly from ATTO-TEC, ensuring a reliable supply.
Black Hole Quenchers : BHQ®-1 - BHQ®-2 - BHQ®-3
Order BHQ®-labeled qPCR probes from Eurogentec for license-free use in diagnostic and commercial applications. BHQ® quenchers are the leading choice for fluorescence-quenched qPCR probes, offering broad absorption from 480 nm to 730 nm for optimal signal suppression.
MGB-Eclipse™ Probes for any applications
By buying your MGB Probes at Eurogentec, you are not limited by any licenses. MGB probes are specific and sensitive probes perfectly suited for patient management and the diagnostic of pathogens.
You can conjugate your MGB probes with a large range of fluorophores from deep blue to infrared.
