How to select your qPCR probe?

  1. Select the probe type

    Double dyes are widely used because they are most efficient and offer sequence specificity SYBR® Green do not. To increase sensitivity and specificity, MGB probes are recommended.
  2. Select your dye

    This step is important to select the most appropriate dye according to your thermocycler. To help you in dye selection, please refer to the compatibility cycler channels versus fluorescent dyes table.
  3. Chose the quencher adapted to your dye

    Each fluorescent Dye emit a specific Wavelength. The Best quenching result is obtained with quenchers that absorb in the same range. To select the best dye/quencher couple, you can consult the dyes & quencher compatibility table.

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