Sirtuin activity measurement
Our SensoLyte® Assay Kit provides a convenient, two-step homogeneous procedure for measuring sirtuin activity using a FRET or a fluorogenic substrate.
Role of Sirtuins
Histone deacetylases (HDAC) act as transcriptional gene repressor through the deacetylation of lysine residues on histone proteins. They are counter-balanced by Histone acetyltransferases (HATs).
Sirtuins (SIRTs) 1 and 2 are signaling proteins possess a deacetylase activity (class III HDACs) that require NAD+ as a cofactor.
Sirtuin 1 (SIRT1) is involved in the regulation of tumor suppressor p53 through deactylation, thereby inhibiting apoptosis. It also represents a target for treatment of age-related diseases and type II diabetes.
Sirtuin 2 (SIRT2) is a cytoplasmic protein involved in regulation of cell cycle, DNA repair and tumorogenesis.