Oligos

3' Amino Modifier C6 - 1 modification

30,00
Check your price
  • Cat.Number : MD-MF006-03004
  • Availability :
    In production

Select specification

Synthesis scale

3' Amino Modifier C6 is a 6 carbon-chain spacer with a branched Methoxy group (-CH2OH) on C1.

The introduction of a primary Amine (NH2) at the 5’-position is used to functionalize the corresponding terminus of the nucleotide for conjugation with e.g. an activated NHS ester or isothiocyanate fluorescent label. Several spacers are available (C3; C6 and C12), all of them are hydrophobic. The available lengths of the spacers are 3, 6, or 12 methylene (CH2) groups between the terminal phosphate and the amino “moiety”.

The 3’-NH2 modification allows the prevention of the 3’>5’ exonuclease degradation of oligonucleotides in biological fluids such as cell culture medium.

C6-dT, dU, Propargyl-dU and Propargylcaproyl-dU amino modifiers are used in post-coupling reactions, for instance, for modifying oligonucleotides that will be printed onto microarrays.

dR-NH2 modification (deoxyribose-NH2) forms abasic sites in the oligonucleotide. It provides the possibility to add more than one label anywhere in the sequence or on either terminus after post-coupling reactions between the Amine group and an activated label. The label is linked to the deoxyribose via a 6-carbon atom spacer which reduces steric hindrance.

Specifications

Chemistry
Spacers Sequence
  • 6 methylene (CH2) groups between the terminal phosphate and the amino “moiety”.
Molecular Mass/ Weight
  • 209,18
Modification
Modification Position
  • 3'
Storage & stability
Resuspension condition
  • - Spin the tube briefly to collect the pellet in the bottom of the tube - Add an appropriate volume of recommended buffer (refer to technical data sheet) - Allow the tube to stand a few minutes - Vortex the tube for 15 seconds
Storage Conditions
  • Dried Oligonucleotides are stable for 18 months at ambiant temperature. Oligonucleotides in solutions are stable for 24 months at -20°C. (Tolerance -20°C± 5°C. ) For more information please refer to the Reconstitution-Storage-Stability page
Activity
Usage
  • Research use

You may also be interested in the following product(s)

Example of a tube containing an Oligonucleotides with 3' Thiol C3  modification

3' Thiol C3 - 1 modification

Cat.Number : MD-MF053-03004
49,00
Example of a tube containing an Oligonucleotides with 5' ATTO 390  modification

5' ATTO 390 - 1 modification

Cat.Number : MD-FL090-39005004
150,00
Picture of a Electroporation cuvettes 1 mm black cap

Electroporation cuvettes 1 mm black cap

Cat.Number : CE-0001-50
140,00