Apelin is the endogenous ligand for the G-protein-coupled APJ receptor. It is produced as a pre-proprotein of 77 amino acids. Upon signal peptide cleavage, the proprotein of 55 amino acids may generate several active fragments: Apelin-36 (36 aa corresponding to the sequence 42-77), Apelin-17 (17 aa corresponding to the sequence 61-77) and Apelin-13 (13 aa corresponding to the sequence 65-77).
Apelin -13 is a vasoactive peptide and one of the most potent endogenous inotropic agents known. It is one of the active peptide fragments resulting from maturation of preproapelin. It is suggested that Apelin-13 could be a competitive agonist to the apelin receptor, which is a class A G-protein coupled receptor and is a putative target for treatment of cardiovascular and metabolic diseases. Apelin-13 may undergo a pyroglutamylation at its N-terminal glutamine residue.