Assay-kits

SensoLyte® 520 ECEs Activity Assay Kit Fluorimetric - 1 kit

462,00
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  • Cat.Number : AS-72243
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Endothelin converting enzymes (ECEs), similar to neprilysin (NEP), belong to the M13 family of zinc metallopeptidases. ECEs (ECE-1/ECE-2) are known as the rate-limiting enzymes in the formation of the vasoconstrictor peptide endothelin-1 (ET-1) from its precursor proendothelin-1. ECE-1 can also degrade amyloid fragments both in vitro and in vivo. Highly homologous and structurally comparable to ECE-1, ECE-2 was shown in homozygous knockout mice to have decreased Abeta degradation and significant elevated Abeta 1-40 and 1-42 in the brain. Some studies indicate that carriers of ECE1 and ECE-2 gene variants may have an increased risk of developing late-onset Alzheimer’s disease (AD). However, the roles of ECE-1 and ECE-2 in AD progression have yet to be fully elucidated.
The SensoLyte® 520 ECEs Activity Assay Kit can be used to detect enzyme activity in biological samples or in purified enzyme preparations. When active ECEs cleave the FRET substrate, it results in an increase of 5-FAM fluorescence, and is monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM shows less interference from autofluorescence of components in biological samples and test compounds. This assay can detect as low as 15.6 ng/mL active ECE-1 and 62.5 ng/mL active ECE-2.

Specifications

Packaging
Kits components
  • Component A: 5-FAM /QXL® 520 ECEs substrate Ex/Em=490 nm/520 nm upon cleavage: 0.5 mM, 50 µL Component B: 5-FAM fluorescence reference standard, Ex/Em=490 nm/520 nm: 0.5 mM, 12 µL Component C: Human recombinant ECE-1: 0.1 mg/mL, 10 µL Component D: 2X Assay Buffer: 20 mL Component E: Inhibitor: 200 µM, 10 µL
Chemistry
UniProt number
  • P42892
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store the kit components A,B,D,E at -20°C. Component C must be stored at -80°C.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 15.6 ng/ml & 62.5 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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