Oligos

dP (pyrimidine hybrid) - 1 modification

93,00
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  • Cat.Number : MD-NB030-IN004
  • Availability :
    In production

Synthesis scale

Universal bases can be used instead of degenerate bases, and have the advantage that the hybridisation probe is not diluted by the non-pairing components of the degeneracy. In this case, the base analogue does not hybridise significantly to the other four bases and makes up for some of the duplex destabilisation by acting as a light intercalating agent. Our available universal nucleosides are 3-nitropyrrole 2’-deoxynucleoside, 5-nitroindole and, finally, P and K nucleoside analogues that are able to hybridise efficiently with respectively both purine (A-G) or pyrimidine (C-T). It is usually not recommended to introduce universal bases in template strands (e.g. PCR primers), as DNA polymerases may dissociate from the template when encoutering these bases.

Specifications

Chemistry
Molecular Mass/ Weight
  • 330,23
Modification
Modification Position
  • 5'
  • IN
Storage & stability
Resuspension condition
  • - Spin the tube briefly to collect the pellet in the bottom of the tube - Add an appropriate volume of recommended buffer (refer to technical data sheet) - Allow the tube to stand a few minutes - Vortex the tube for 15 seconds
Storage Conditions
  • Dried Oligonucleotides are stable for 18 months at ambiant temperature. Oligonucleotides in solutions are stable for 24 months at -20°C. (Tolerance -20°C± 5°C. ) For more information please refer to the Reconstitution-Storage-Stability page
Activity
Usage
  • Research use

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