Labeling-detection

HiLyte™ Fluor 488 C2 maleimide - 1 mg

166,00
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  • Cat.Number : AS-81164
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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HiLyte™ Fluor 488 C2 maleimide is an excellent thiol-reactive fluorescent labeling dye that generates the protein conjugates far superior to those of fluorescein derivatives such as FITC. Not only are HiLyte™ Fluor 488 conjugates significantly brighter than fluorescein conjugates, they are much more photostable. Also, fluorescence of HiLyte™ Fluor 488 fluorophore is independent of pH from 4 to 10. This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only above pH 10. Extinction Coefficient (M-1cm-1): 70,000; Fluorescence quantum yield: 0.91; Fluorescence Life Time (ns): 4.1

Specifications

Chemistry
Molecular Mass/ Weight
  • 567.6
Properties
Absorbance (nm)
  • 502
Emission (nm)
  • 527
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO: Prepare 10 mg/ml dye stock solution in high quality anhydrous DMSO. Completely dissolve all dye content by vortexing. Dye solution should be prepared fresh, immediately before an experiment. Longterm storage in solution form may reduce dye activity. Any solution containing the dye should be protected from light.
Storage Conditions
  • -20°C desiccated and protected from light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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