PCR & qPCR

Hot Diamond Taq® DNA Polymerase

180,00
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  • Cat.Number : TAQ-I033-1000-
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Select specification

Size

Quantity

Hot Diamond Taq® polymerase is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5’-3’ synthesis of DNA with no detectable 3’-5’ proof reading exonuclease activity. This enzyme has the “extendase” activity allowing TA cloning.

Hot Diamond Taq® polymerase exhibits unique Hot Start characteristics and represents a completely new “Hot Start concept”. Hot start characteristics are not accomplished through chemically modification nor a blocking antibody but a proprietary agent prevents non-specific polymerization; thereby preventing primer-dimer formation and increasing the PCR yield of specific products. The enzyme needs very short activation time (100 % activated during the first PCR cycle) but is compatible with all existing protocols (from 20 sec. to 15 min. at 95 °C).

Hot Diamond Taq® polymerase is particularly recommended for PCR applications that require ultra low levels of bacterial & fungal DNA in Diagnostic and demanding Research fields but also for amplification of long and difficult templates.

Hot Diamond Taq® polymerase can be ordered according to your needs:
Under IVD process (please enquire):
- Shipment on dry ice
- Hard copy CoA
- Full traceability from production, storage to shipment
- Tracking number sent to the customer the day of the shipment
- Customized Fill & Finish. On request Hot Diamond Taq® can be produced with an activity from 5 to 200U/µl; a glycerol level from 1 to 50 %
Under Research process:
- Shipment at room temperature
- Full traceability from production to storage of the product

Specifications

Packaging
Kits components
  • TAQ-I033-100-contains: - One tube (20 µl) of Hot Diamond Taq® DNA Polymerase (concentration > 5 U/µl) - One tube (1 ml) of reaction buffer 10x (150 mM Tris-HCl pH 8.0 (at 25°C), 500 mM KCl and stabilizer) - One tube (1ml) of MgCl2 solution (25 mM MgCl2). TAQ-I033-1000- contains: - One tube (200 µl) of HGS Diamond Taq® DNA Polymerase (concentration > 5 U/µl) - One bottle (6 ml) of reaction buffer 10x (150 mM Tris-HCl pH 8.0 (at 25°C), 500 mM KCl and stabilizer) - One bottle (6 ml) of MgCl2 solution (25 mM MgCl2). TAQ-I033-5000 contains: - One tube (1 ml) of HGS Diamond Taq® DNA Polymerase (concentration > 5 U/µl) - One bottle (30 ml) of reaction buffer 10x (150 mM Tris-HCl pH 8.0 (at 25°C), 500 mM KCl and stabilizer) - One bottle (30 ml) of MgCl2 solution (25 mM MgCl2). TAQ-I033-25000- contains: - Five tubes (1 ml each) of Hot Diamond Taq® DNA Polymerase (concentration > 5 U/µl) - Five bottles (30 ml each) of reaction buffer 10x (150 mM Tris-HCl pH 8.0 (at 25°C), 500 mM KCl and stabilizer) - Five bottles (30 ml each) of MgCl2 solution (25 mM MgCl2)
Chemistry
Molecular Mass/ Weight
  • approx. 95 kDa
Properties
Color
Quantity & Purity
Concentration
  • >= 5 U/µl
Purification Method
  • SDS-PAGE
Purity
  • > 98%
Storage & stability
Form
  • In solution
Storage Buffer
  • 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5 % (v/v) Nonidet P40, 0.5 % (v/v) Tween 20, 50 % (v/v) glycerol and stabilizer, pH 8.0 (19 °C).
Storage Conditions
  • Storage at -20 °C is recommended 
Activity
Template type
  • DNA
Unit definition
  • One unit is defined as the amount of enzyme that incorporates, after activation step, 10 nmoles of dNTPs into acid insoluble form in 30 minutes at 74 °C.
Usage
  • Diagnostics
  • Research use
Source
Host
Source / Species
  • Thermus aquaticus
Codes
Code Nacres
  • NA.55

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