Amplification technology
Takyon® Etheris
qPCR Master Mix
Takyon® Etheris is formulated with ultra-purified components to minimize residual E. coli DNA left from the manufacturing process. This ensures clean amplification and accurate microbial detection even at low target abundance.
Tailored for microbial DNA
quantification and identification
Optimized for both Probe
and SYBR® Green-based assays
Probe qPCR assays
Takyon® Etheris Probe is provided as a 2X Clear Master Mix, optimized for high specificity and accuracy in microbial DNA detection. Designed for sensitive workflows, it delivers clean amplification with minimal background signal.
SYBR® Green qPCR assays
Takyon® Etheris SYBR® is provided as a 2X Blue Master Mix, ideal for rapid and reliable DNA detection. Designed for everyday microbial qPCR assays, its blue-colored format simplifies setup and minimizes pipetting errors.
Confident Assay Results
Trustworthy results, low background to reduce false positives.
Versatile qPCR Master Mixes
Compatible with all thermocyclers, for probe and SYBR®-based detection.
Performant Probe Assays
High sensitivity, inhibitor resistance, low-copy detection.
Clean manufacturing for trusted qPCR results
Takyon® Etheris is assembled in ISO-classified cleanrooms using purified components with minimal residual host-derived DNA to generate clean NTC.
Each batch undergoes strict quality control, including background DNA testing, to ensure:
- Low bacterial nucleic acid content
- Consistent lot-to-lot performance
- Reliable results in sensitive qPCR assays
Takyon® Etheris technical details
| Takyon® Etheris for SYBR®-based assays | Takyon® Etheris for Probe-based assays | |
|---|---|---|
| 2X Master Mix | Blue The Blue non-fluorescent dye improves contrast, making Master Mix dispensing easier and more reliable. |
Clear Compatible with Takyon® Blue Additive |
| Hot Start Takyon® fusion DNA polymerase* | V | V |
| Compatible with all qPCR instruments Includes separate ROX™ passive reference tube. |
V | V |
| qPCR Protocol and Kit Components | ||
*Ultra-purified Taq polymerase free of residual host DNA
For any assay aimed at
detecting microbial DNA
Environmental microbiology
Ensure accurate detection of microbial DNA in soil, plant and animal tissues even in inhibitor-rich samples for environmental monitoring and contamination control.
Water quality monitoring
Analyze drinking water, process water or wastewater for microbial presence using ultra-sensitive qPCR mixes with minimized reagent-derived DNA background.
Food safety
& quality
Detect foodborne pathogens (e.g., E. coli, Listeria, Salmonella) and monitor microbial contaminants to validate hygiene and HACCP procedures in food, beverage and dairy production.
Microbial diagnostics
Enable accurate infectious disease monitoring in human samples and support assay development or quality control of diagnostic tests.
Microbiome analysis
Quantify microbial DNA in swabs or feces without interference, enabling clean baselines for 16S or metagenomics workflows.
DNA
barcoding
Identify and track microbial species through highly specific amplification for food authenticity, biodiversity studies, or supply chain traceability.
Benchmarking Takyon® Etheris
Takyon® Etheris removes background signals
Fig. 1A. Amplification curves with (line) or without (dotted line) E.coli gDNA (500 pg) in a qPCR reaction using either Takyon® Etheris Probe (orange) or a competitor (blue) kit. Each assay was performed in technical triplicates. All reactions were run according to the respective manufacturer’s protocols.
Fig. 1B. Mean Cq values obtained with Takyon® Etheris Probe qPCR Master Mix (orange) and a competitor kit (blue) in a 23S-targeted qPCR assay. Each reaction was performed in triplicate under identical assay conditions, either with 500 pg of E. coli gDNA or as a no template control (NTC). All reactions were run according to the respective manufacturer’s protocols.
The results indicate that Takyon® Etheris delivers higher sensitivity and stronger amplification signals, with no detectable signal from residual bacterial gDNA. By contrast, the competitor kit produced a Cq signal in the no template control (NTC), demonstrating contamination from residual host DNA.
Takyon® Etheris outperforms specialized competitor’s reagents
Compared to the competitor kit, Takyon® Etheris demonstrates improved sensitivity and stronger fluorescence signal intensity across tested targets, proving its value in multiplex conditions.
Fig. 2A. Amplification curves obtained with Takyon® Etheris Probe qPCR Master Mix (orange) and a competitor kit (blue) in a triplex assay targeting 18S, B2MG, and ACTB genes. Each reaction was performed in triplicate using 5 ng of DNA template according to the respective manufacturer’s protocols.
Sensitivity comparison of Takyon® Etheris vs competitor qPCR kits
Fig. 2B and Fig. 2C : Amplification curves obtained with Takyon® Etheris Probe qPCR Master Mix (orange) and a competitor kit (blue) for the amplification of the human ACTB (Fig. 2B) and 18S (Fig. 2C) genes. Five serial dilutions of genomic DNA were tested: 50 ng, 5 ng, 0.5 ng, 50 pg, and 5 pg. Each point represents the mean Cq value of triplicate reactions performed under manufacturer’s protocol instructions.
The data demonstrate that Takyon® Etheris delivers superior sensitivity and consistency across both ACTB and 18S targets, maintaining efficient amplification and reliable detection down to 5 pg of human gDNA. In contrast, the competitor kit exhibits delayed amplification and higher mean Cq values, indicating lower sensitivity and reduced amplification efficiency across gene targets.
Takyon® Etheris maintains the performance of the Takyon® range
Takyon® Etheris and Takyon® Ultra exhibit similar sensitivity and amplification performance across all three targets at 50 pg of human gDNA. Mean Cq values are closely aligned between the two formulations, confirming their equivalent efficiency in detecting low-copy templates.
Fig. 3. Amplification curves obtained with Takyon® Etheris Probe qPCR Master Mix (orange) and Takyon® Ultra Probe qPCR Master Mix (blue) for amplification of three human targets: 18S, ACTB, and B2MG. Each reaction was performed in triplicate using 50 pg of human genomic DNA as template.
FAQs
What makes Takyon® Etheris different from other qPCR Master Mixes?
Takyon® Etheris is specifically formulated with ultra-purified components to minimize residual host-derived DNA, significantly reducing background signals and risk of false positives.
In which applications is Takyon® Etheris recommended?
Takyon® Etheris is recommended for microbial DNA qPCR detection in environmental monitoring, water and food safety testing, microbiome qPCR studies, clinical and diagnostic microbiology, and quality control of microbial contaminants.
Can Takyon® Etheris be used for quality control (QC) testing?
Yes. Its clean formulation and consistency make it well-suited for microbial QC workflows in manufacturing, diagnostics, and research environments.
Can I use Takyon® Etheris for very low copy number detection?
Absolutely. Its high sensitivity and clean formulation make it ideal for detecting low microbial loads, ensuring trust in your negative controls and positive sample results.
Is Takyon® Etheris only recommended for qPCR with microbial targets?
No. While Takyon® Etheris is optimized for microbial DNA qPCR detection, it is also a versatile Master Mix suitable for any qPCR target. Its ultra-purified formulation and high-performance polymerase make it a reliable choice for in vitro diagnostic assays, CE-marked kits, and other molecular testing applications where quality and accuracy is critical.
Is Takyon® Etheris compatible with all qPCR instruments?
Yes. It is universally compatible, with a separate ROX™ passive reference dye tube included by default for platforms that require it.
Does Takyon® Etheris work with both SYBR® and probe-based assays?
Yes. It is available in two versions:
- Takyon® Etheris SYBR® 2X Master Mix Blue : cost-effective, fast screening with visual pipetting aid
- Takyon® Etheris Probe 2X Master Mix: for high-specificity probe-based assays.
Explore the full Takyon® range
We offer a complete selection of Takyon® kits for both qPCR and RT-qPCR applications.
Whether you're working with DNA or RNA templates, our off-the-shelf Master Mixes and Core Kits
are designed to meet a wide variety of experimental and diagnostic needs.
Each kit includes high-performance polymerases and optimized buffer to deliver reliable
amplification across all qPCR instruments, even with complex samples or low-copy target conditions.
Standard Takyon®
Reliable performance for everyday qPCR assays.
Cost-effective, robust, and optimized for routine DNA and RNA quantification.
Takyon® Ultra
Enhanced mix for complex samples.
High sensitivity, improved inhibitor tolerance for fast, reproducible results.
Takyon® Etheris
Ultra-clean mix for microbial detection.
Minimizes residual host DNA, ensuring accurate results and clean NTC.
Services for Nucleic Acid
Amplification (NAAmp)
In addition to our Takyon® qPCR kits, we offer a full panel of services to support your assay development
and testing workflows. These include the custom synthesis of primers and probes, tailor-made
qPCR kit formulations when off-the-shelf solutions don’t meet your needs, in-house assay
and Lab developed test preparation for routine testing, and OEM manufacturing services for diagnostic kit providers.
