Protein

Human MMP-1 - 100 µL

108,00
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  • Cat.Number : AS-72004
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    In stock
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Matrix metalloproteinases (MMP’s) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. MMP-1 (collagenase-1) is involved in tumor development and metastasis and rheumatoid arthritis. It is proposed as a therapeutic target for these diseases. Native pro-MMP-1 is prepared from culture medium of human rheumatoid synovial fibroblasts. MMP-1 is secreted as pro-enzyme, which consists of a propeptide of 80 amino acids, a catalytic domain of 162 amino acids, a 16-residue linker region, and a hemopexin domain of 189 amino acids. The native pro-MMP-1 has a major Mr 52-kDa unglycosylated and a minor Mr 57-kDa glycosylated form. The proteolytic activation of the 57/52-kDa species will form 47/42-kDa active collagenase, and a 22-kDa C-terminal fragment

The apparent Mr on SDS-PAGE is approximately 56kDa/52 kDa. The pro-MMP-1 can be fully activated by incubating with 1 mM APMA at 37°C for 3 hr. Its activity can be measured by FRET peptides. 10-20 ng of enzyme is sufficient for FRET-based assay

Specifications

Chemistry
UniProt number
  • P03956 (pro-MMP-1)
Molecular Mass/ Weight
  • 56 kDa
Modification
Conjugation
  • Unconjugated
Quantity & Purity
Concentration
  • 10 µg/ml
Storage & stability
Form
  • In solution
Storage Buffer
  • 0.1M Tris, pH 7.5, 0.1M NaCl, 10 mM CaCl2, 0.05% Brij 35, 2mM sodium azide, 1 mg/mL BSA
Storage Conditions
  • Store at -80 ºC. Avoid repeated freeze-thaw cycles.
Activity
Application
Biomarker Target
Unit definition
  • One unit of protease hydrolyzes 1 picomole of 5-FAM-Pro-Leu-Ala-Nva-Dap (QXL™520 )-Ala-Arg-NH2 per minute at pH 7.5 at 25° C.
Specificity
  • Human MMP-1
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Source / Species
  • human
Codes
Code Nacres
  • NA.27

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Citations

A bioresponsive hydrogel tuned to chondrogenesis of human mesenchymal stem cells

FASEB J . 2011 Jan 31 ; 25(5) 1486 | DOI : 10.1096/fj.10-165514

  • C. Bahney
  • et al

Cleavage Site Specificity and Conformational Selection in Type I Collagen Degradation

Biochemistry . 2011 May 18 ; 49(19) 4147 | DOI : 10.1021/bi9021473

  • R. Salsas-Escat
  • et al

Development of a Gene Therapy Virus with a Glucocorticoid-Inducible MMP1 for the Treatment of Steroid Glaucoma

Invest Ophthalmol Vis Sci. . 2010 Jan 20 ; 51(6) 3029 | DOI : 10.1167/iovs.09-4918

  • M. Spiga
  • et al