Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. MMP-2 (72-kDa gelatinase-A) is involved in tumor development and metastasis. It is proposed as a therapeutic target for cancer.
Recombinant human MMP-2 was expressed as a pro-enzyme from its DNA sequence, transfected into CHO cells. The pro-MMP-2 can be fully activated by incubating with 1 mM APMA at 37°C for 20 min to 1 hr. Its activity can be measured in FRET-based enzymatic assays. 10-20 ng of enzyme is sufficient for FRET-based assay.
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50mM Tris-HCl at pH 7.5, 0.2M NaCl, 5mM CaCl2, 1uM ZnCl2 , 0.05% Brij35 and 1mg/ml BSA.
Store at -80 ºC. Avoid repeated freeze-thaw cycles.