Protein

iD BlueLadder Revealer - 50 µl

81,00
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  • Cat.Number : ID-MWLRV1-050
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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Quantity

The iD BlueLadder Revealer is a mouse monoclonal antibody targeting the blue dye of prestained protein weight ladders. In combination with an anti-mouse secondary antibody approprietely conjugated, it allows visualizing the marker blue bands throughout the whole process of western blotting from gels to membranes and up to X-Ray films. This makes the positioning of the marker on the blot very easy and highly accurate.

Instruction for Western Blotting application:
Run an SDS-PAGE gel with a pre-stained molecular weight marker containing blue bands and transfer the proteins to a membrane
Block the membrane using an appropriate blocking solution e.g. iD Blocking Kit (ID-WCBLO1-001; Eurogentec)
Incubate the membrane with a mix of primary antibodies containing:
- A mouse primary antibody specific to your protein (for dilution, please refer to the instructions from the manufacturer);
- The iD BlueLadder Revealer (mouse mAb; recommended dilution : 1/2000)
Incubate the membrane with an anti-mouse secondary antibody, conjugated.
Perform the detection according to the used conjugate

Note: if HRP is used, a colorimetric or chemiluminescent detection can be performed using respectively the iD TMB Substrate Kit (ID-SUTMB1) or iD HRP Substrate Kit (ID-SUHRP1) (Eurogentec).
The iD BlueLadder Revealer and the iD BlueLadder Revealer-HRP are compatible with the iD Western 1H Detection Kits (Eurogentec).

IMPORTANT NOTE: use the iD BlueLadder Revealer-HRP instead of the iD BlueLadder Revealer:
- If the primary antibody specific to your protein is HRP-conjugated
- If the primary antibody specific to your protein was not raised in mouse

Specifications

Packaging
Kits components
  • 1 vial of 50 µL (100 µg) iD BlueLadder Revealer - Monoclonal Antibody
Modification
Conjugation
  • Unconjugated
Quantity & Purity
Concentration
  • 2 µg/µl
Storage & stability
Form
  • In solution
Storage Buffer
  • iD BlueLadder Revealer is delivered in PBS containing 0.05% azide.
Storage Conditions
  • Store at -20°C. Avoid multiple freeze-thaw cycles.
Activity
Application
Specificity
  • This antibody is specific to the blue dye present in prestained protein weight markers. It does not react with human, mouse, rat, chicken, hamster, monkey, yeast and E. coli proteins.
Clonality
Usage
  • Research use

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