iD BlueLadder Revealer - 50 µl

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  • Cat.Number : ID-MWLRV1-050
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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The iD BlueLadder Revealer is a mouse monoclonal antibody targeting the blue dye of prestained protein weight ladders. In combination with an anti-mouse secondary antibody approprietely conjugated, it allows visualizing the marker blue bands throughout the whole process of western blotting from gels to membranes and up to X-Ray films. This makes the positioning of the marker on the blot very easy and highly accurate.

Instruction for Western Blotting application:
Run an SDS-PAGE gel with a pre-stained molecular weight marker containing blue bands and transfer the proteins to a membrane
Block the membrane using an appropriate blocking solution e.g. iD Blocking Kit (ID-WCBLO1-001; Eurogentec)
Incubate the membrane with a mix of primary antibodies containing:
- A mouse primary antibody specific to your protein (for dilution, please refer to the instructions from the manufacturer);
- The iD BlueLadder Revealer (mouse mAb; recommended dilution : 1/2000)
Incubate the membrane with an anti-mouse secondary antibody, conjugated.
Perform the detection according to the used conjugate

Note: if HRP is used, a colorimetric or chemiluminescent detection can be performed using respectively the iD TMB Substrate Kit (ID-SUTMB1) or iD HRP Substrate Kit (ID-SUHRP1) (Eurogentec).
The iD BlueLadder Revealer and the iD BlueLadder Revealer-HRP are compatible with the iD Western 1H Detection Kits (Eurogentec).

IMPORTANT NOTE: use the iD BlueLadder Revealer-HRP instead of the iD BlueLadder Revealer:
- If the primary antibody specific to your protein is HRP-conjugated
- If the primary antibody specific to your protein was not raised in mouse


Kits components
  • 1 vial of 50 µL (100 µg) iD BlueLadder Revealer - Monoclonal Antibody
  • Unconjugated
Quantity & Purity
  • 2 µg/µl
Storage & stability
  • In solution
Storage Buffer
  • iD BlueLadder Revealer is delivered in PBS containing 0.05% azide.
Storage Conditions
  • Store at -20°C. Avoid multiple freeze-thaw cycles.
  • This antibody is specific to the blue dye present in prestained protein weight markers. It does not react with human, mouse, rat, chicken, hamster, monkey, yeast and E. coli proteins.
  • Research use
Code Nacres
  • NB.22

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