N4-Et-dC - 1 modification

It was found that the N4-ethyl analogue (N4-Et-dC) hybridises specifically to natural dG but the stability of the base pair is reduced to about the level of an AT base pair. In a series of probes whose GC content ranged from 0 to 100%, the range in Tm values when N4-Et-dC was used was only 7°C; when dC was used, that range was 39°C. The N4-ethyl analog (N4-EtdC) could be used to normalise the stability of the GC and AT base pairs, crucial in any technique that involves hybridisation of multiple sequences simultaneously, as in DNA chip and reverse hybridisation technologies.

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