Oligos

N4-Et-dC - 1 modification

101,00
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  • Cat.Number : MD-NB180-IN004
  • Availability :
    In production

Synthesis scale

It was found that the N4-ethyl analogue (N4-Et-dC) hybridises specifically to natural dG but the stability of the base pair is reduced to about the level of an AT base pair. In a series of probes whose GC content ranged from 0 to 100%, the range in Tm values when N4-Et-dC was used was only 7°C; when dC was used, that range was 39°C. The N4-ethyl analog (N4-EtdC) could be used to normalise the stability of the GC and AT base pairs, crucial in any technique that involves hybridisation of multiple sequences simultaneously, as in DNA chip and reverse hybridisation technologies.

Specifications

Chemistry
Molecular Mass/ Weight
  • 317,42
Modification
Modification Position
  • IN
  • 5'
Storage & stability
Resuspension condition
  • - Spin the tube briefly to collect the pellet in the bottom of the tube - Add an appropriate volume of recommended buffer (refer to technical data sheet) - Allow the tube to stand a few minutes - Vortex the tube for 15 seconds
Storage Conditions
  • Dried Oligonucleotides are stable for 18 months at ambiant temperature. Oligonucleotides in solutions are stable for 24 months at -20°C. (Tolerance -20°C± 5°C. ) For more information please refer to the Reconstitution-Storage-Stability page
Activity
Usage
  • Research use

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