R-Phycoerythrin (R-PE), a fluorescent protein from phycobiliprotein family, is isolated from red algae. Its primary absorption peak is at 565 nm with secondary peaks at 496 and 545 nm. The broad excitation spectrum provides the advantage for multi-color immunofluorescent staining or cell sorting. R-PE consists of α, β and γ subunits and is present as (αβ)6γ. R-PE and the closely related B-PE are the most intensely fluorescent phycobiliproteins having orange fluorescence. They are significantly brighter and more photostable than conventional organic fluorophores.
Before use, centrifuge the R-PE suspension at 10,000g for 10 min at 4°C. Discard the supernatant. Resuspend the pellet (R-PE) into the desired buffer. Desalt the sample using either Sephadex G-25 or dialysis.
Sodium phosphate buffer, pH 7.0 with ammonium sulfate
Store at 4°C and protect from light. DO NOT FREEZE